可变剪切和小鼠卵泡组装过程阶段转换相关
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南京医科大学生殖医学国家重点实验室

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国家重点基础研究发展计划(973计划)


Alternative splicing associated with cellular stage transitions during follicle assembly in mice
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State Key Laboratory of Reproductive Medicine,Nanjing Medical University

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    摘要:

    摘 要: 目的:通过对新生小鼠卵巢单个生殖细胞转录组数据进行挖掘,探究可变剪切事件是否参与调控小鼠卵泡组装过程的阶段转换。方法:基于小鼠单个生殖细胞转录组数据,用生物信息学方法分析卵泡组装过程3个细胞发育阶段的可变剪切事件及阶段转换之间的差异可变剪切事件,并用RT-PCR验证差异可变剪切相关同源异构体的变化。结果:在小鼠卵泡组装3个发育阶段转换之间均检测到差异可变剪切事件,其中卵泡生成阶段可变剪切事件以及包囊破裂到卵泡生成时期的差异可变剪切事件富集最多,RT-PCR验证了减数分裂相关同源异构体的变化。结论:可变剪切和小鼠卵泡组装细胞发育阶段转换相关,调控生殖细胞到卵母细胞转变过程。

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    Abstract: Objective: To investigate whether alternative splicing regulate the stage transition of follicle assembly in mice, the transcriptome data of single germ cells in new born mouse ovary were analyzed. Methods: Bioinformatics analysis were performed on transcriptome data of single germ cells in mice to detect alternative splicing events in three cell development stages during follicle assembly and differential alternative splicing events between stage transitions. RT-PCR was used to validate dynamic stage transition related isoform expression. Results: This study detected abundant differential alternative splicing events between stage transitions during follicle assembly in mice. Then detected the most enriched splicing events in follicle stage and differential splicing events between cyst break down and follicle stage. The results of RT-PCR validated the presence of isoforms of meiosis related genes and found their shifts of alternative isoform usage at stage transitions. Conclusion: Alternative splicing was associated with cell development stage transitions during follicle formation in mice, regulating the transformation from germ cell to oocytes.

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  • 收稿日期:2023-01-04
  • 最后修改日期:2023-03-21
  • 录用日期:2023-07-13
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