［Abstract］ Objective: To determine the expression level of Vacuolar Protein Sorting 13 Homolog A (VPS13A) during cell differentiation of 3T3-L1; Explore the effects of VPS13A on 3T3-L1 cells Method: Western blotting and real-time PCR were used to detect the expression changes of VPS13A during the differentiation of 3T3-L1 cells, and CRISPR (Clustered regularly interspaced short palindromic repeats) /Cas9(CRISPR associated with 9) system to establish Stable cell lines that knockdown VPS13A. Finally, the effect of VPS13A knockdown on 3T3-L1 cells differentiation was detected by western blotting and Oil red staining. Results: The expression of VPS13A was downregulated in the early stage and upregulated in the later stage of 3T3?L1 cells differentiation. Lipid droplets and the expression levels of gene involved in differentiation were increased in VPS13A knockdown 3T3-L1 cells. Conclusion: VPS13A expression is modulated by cell differentiation status in 3T3-L1 cells. Knockdown of VPS13A enhances differentiation of 3T3-L1 cells.