VPS13A在3T3-L1 脂肪细胞分化过程中的表达及调控研究
DOI:
作者:
作者单位:

南京医科大学罕见代谢性疾病研究重点实验室

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(32130050;3220110126); 南京医科大学科技发展基金-面上项目(编号:2016NJMU004)


Expression and Regulation of VPS13A in 3T3-L1 adipocytes during differentiation
Author:
Affiliation:

Key Laboratory of Rare Metabolic Disease,Department of Molecular Biology and Biochemistry,Nanjing Medical University,The Key Laboratory of Human Functional Genomics of Jiangsu Province

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    [摘 要] 目的:明确囊泡分选蛋白13A(Vacuolar Protein Sorting 13 Homolog A,VPS13A)在小鼠胚胎成纤维细胞(3T3-L1)分化过程中的表达水平;探索VPS13A对3T3-L1细胞的影响。方法:利用免疫印迹法(Western blot)及荧光定量PCR(Q?PCR)法检测3T3-L1细胞在分化过程中VPS13A的表达,利用成簇规律间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)/CRISPR相关蛋白9(CRISPR associated protein 9,Cas9)系统构建VPS13A稳定敲降细胞系,Western blot和油红(Oil Red O)染色检测VPS13A敲降后对3T3-L1细胞分化的影响。结果:VPS13A的表达在3T3-L1分化过程的早期降低,在分化的后期升高。敲降VPS13A后,3T3-L1细胞中脂滴增多,参与调控脂肪细胞分化基因的表达水平升高。结论:在3T3-L1细胞中VPS13A的表达水平在分化过程中被调控,敲降VPS13A可以促进3T3-L1细胞的分化成熟。

    Abstract:

    [Abstract] Objective: To determine the expression level of Vacuolar Protein Sorting 13 Homolog A (VPS13A) during cell differentiation of 3T3-L1; Explore the effects of VPS13A on 3T3-L1 cells Method: Western blotting and real-time PCR were used to detect the expression changes of VPS13A during the differentiation of 3T3-L1 cells, and CRISPR (Clustered regularly interspaced short palindromic repeats) /Cas9(CRISPR associated with 9) system to establish Stable cell lines that knockdown VPS13A. Finally, the effect of VPS13A knockdown on 3T3-L1 cells differentiation was detected by western blotting and Oil red staining. Results: The expression of VPS13A was downregulated in the early stage and upregulated in the later stage of 3T3?L1 cells differentiation. Lipid droplets and the expression levels of gene involved in differentiation were increased in VPS13A knockdown 3T3-L1 cells. Conclusion: VPS13A expression is modulated by cell differentiation status in 3T3-L1 cells. Knockdown of VPS13A enhances differentiation of 3T3-L1 cells.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2023-03-14
  • 最后修改日期:2023-04-19
  • 录用日期:2023-08-09
  • 在线发布日期:
  • 出版日期: