靶向MPO的次氯酸荧光探针用于细胞成像
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南京医科大学

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国家自然科学基金


Hypochloric acid fluorescence probe targeting MPO for cell imaging
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National Natural Science Foundation of China

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    摘要:

    目的:合成靶向髓过氧化物酶(myeloperoxidase, MPO)的次氯酸(hypochloric acid, HClO)荧光探针并评估其HClO示踪活性。方法:以硅罗丹明为荧光团,KYC为MPO靶向基团合成SiRho-GABA-KYC,用紫外分光光度计和荧光分光光度计检测其紫外光谱和荧光光谱以及pH稳定性和选择性,用CCK8法检测细胞毒性,用共聚焦显微镜进行细胞荧光成像,统计各实验数据,作图分析。结果:合成了SiRho-GABA-KYC,对HClO响应高(荧光增强超过40倍),选择性好,细胞毒性小,可示踪Hela细胞中HClO浓度的变化。结论:探针SiRho-GABA-KYC可用于检测HClO浓度进而反映MPO催化活性。

    Abstract:

    Objective: This study aims to synthesize hypochloric acid (HClO) fluorescent probes targeting myeloperoxidase (MPO) and evaluate the activity of its HClO tracing activity. Methods: Si-rhodamine was used as the fluorophore with KYC as MPO targeting group to synthesize SiRho-GABA-KYC. The UV spectrum, fluorescence spectrum, pH stability and selectivity were detected with UV spectrophotometer and fluorescence spectrophotometer. Cytotoxicity was detected with CCK8 method, and cell fluorescence imaging was performed by confocal microscopy, collected experimental data, ploted and analysed. Results: SiRho-GABA-KYC was synthesized, with high response to HClO (fluorescence enhancement>40 times), good selectivity, and low cytotoxicity, which can trace changes in HClO concentration in Hela cells. Conclusion: The probe SiRho-GABA-KYC can be used to detect HClO concentration, which in turn reflects MPO catalytic activity.

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  • 收稿日期:2023-06-02
  • 最后修改日期:2023-07-24
  • 录用日期:2023-10-18
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