Objective: This study aims to synthesize hypochloric acid (HClO) fluorescent probes targeting myeloperoxidase (MPO) and evaluate the activity of its HClO tracing activity. Methods: Si-rhodamine was used as the fluorophore with KYC as MPO targeting group to synthesize SiRho-GABA-KYC. The UV spectrum, fluorescence spectrum, pH stability and selectivity were detected with UV spectrophotometer and fluorescence spectrophotometer. Cytotoxicity was detected with CCK8 method, and cell fluorescence imaging was performed by confocal microscopy, collected experimental data, ploted and analysed. Results: SiRho-GABA-KYC was synthesized, with high response to HClO (fluorescence enhancement>40 times), good selectivity, and low cytotoxicity, which can trace changes in HClO concentration in Hela cells. Conclusion: The probe SiRho-GABA-KYC can be used to detect HClO concentration, which in turn reflects MPO catalytic activity.