Objective: To investigate the correlation between lymphocyte subsets and cytokine expression levels and blood cell count and mutation types in peripheral blood of newly diagnosed AML patients with FLT3 mutations. Methods: A total of 168 AML patients with FLT3 mutations were included in the study. FLT3 mutation types were further classified, and lymphocyte subsets, cytokines and blood cell count in peripheral blood of patients before treatment were collected to analyze their correlation. Results: Compared with the healthy control group, the expression levels of CD4, IL-6, and IL-10 in peripheral blood of patients with FLT3 mutations were increased before treatment, while the expression levels of CD8, CD16+CD56, IL-2, TNF, and IFN-γ were decreased. According to leukocyte count, the distributions of CD3, CD4, CD8, CD19, IL-4 and IL-10 were different among the groups (P=0.0385, P=0.0235, P=0.0338, P=0.0084, P=0.0478, P=0.0241). According to platelet count, the distributions of CD19, CD16+CD56 were different among groups (P=0.0298, P=0.0453). It can be divided into FLT3-ITD+、FLT3-TKD+、FLT3-（ITD++TKD+）and FLT3-（ITD-+TKD-）. The distributions of CD3, CD8, CD19, IL-10 and TNF were different among the four groups (P=0.0460, P=0.0484, P=0.0412, P=0.0415, P=0.0126). Conclusion: The expression levels of lymphocyte subsets and cytokines in AML patients with FLT3 mutations were different compared to control group. Blood cell count and FLT3 mutation types may influence their expression level.