迷迭香酸-锌配合物抗氧化和抗菌性研究
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1.南京医科大学口腔医学院;2.南京医科大学附属口腔医院

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江苏省重点研发计划(社会发展)项目(BE2022797);江苏省大学生创新训练重点项目(202210312004Z)


Study on antioxidant and antimicrobial properties of rosmarinic acid-zinc complexes
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1.School of Stomatology, Nanjing Medical University;2.the Affiliated Stomatological Hospital of Nanjing Medical University

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Jiangsu Key R&D Program (Social Development) Project (BE2022797); Jiangsu Key Project on Innovation Training for University Students (202210312004Z)

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    摘要:

    目的:研究迷迭香酸(rosmarinic acid,RA)和锌(zinc,Zn)离子络合后的结构、抗氧化性、抗菌性和生物相容性。方法:利用扫描电子显微镜、红外和紫外-可见光谱分析迷迭香酸锌(II)配合物的结构。通过细胞增殖实验比较迷迭香酸和迷迭香酸锌(II)配合物对成骨细胞增殖活性影响,通过铁离子还原抗氧化能力实验和2,2-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt)自由基(ABTS+)清除实验分析两者的抗氧化性,最小抑菌浓度(MIC)法比较两者对金黄色葡萄球菌的抑制活性。结果:成功合成了迷迭香酸-锌(II)配合物(RA-Zn)。到第五天,RA-Zn促进成骨细胞增殖作用优于RA(P<0.05),200μmol/L RA-Zn比RA清除ABTS+阳离子自由基和还原Fe3+水平更高(P<0.05)。高于400μmol/L的RA抑制金黄色葡萄球菌活性(P<0.05),而RA-Zn从浓度25μmol/L开始,抑菌活性有统计学差异(P<0.05)。结论:迷迭香酸和锌离子络合后抗氧化性和抗菌性增强,具有促进成骨细胞增殖作用。

    Abstract:

    Objective:To investigate the structure, antioxidant, antimicrobial and biocompatibility of rosmarinic acid and zinc (II) complexes. Methods:The structures of zinc (II) complexes with rosmarinic acid were analyzed by scanning electron microscopy, infrared and ultraviolet-visible spectroscopy. The effects of rosmarinic acid and zinc (II) complexes with rosmarinic acid on the proliferative activity of osteoblasts were compared by cell proliferation assay, the antioxidant properties of both of them were analyzed by ferric ion reducing antioxidant power assay and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt free radical (ABTS+) scavenging assay. The minimum inhibitory concentration (MIC) assay compared the inhibitory activities of the two against Staphylococcus aureus. Results:The rosmarinic acid-zinc (II) complex (RA-Zn) was successfully synthesized, and by the fifth day, the osteoblast proliferation-promoting of RA-Zn was superior to that of RA (P<0.05). 200 μmol/L RA-Zn scavenged higher levels of ABTS+ cationic free radicals and reduced Fe3+ than RA (P<0.05). RA above 400 μmol/L inhibited Staphylococcus aureus activity (P<0.05), whereas there was a statistically significant difference in the inhibitory activity of RA-Zn from a concentration of 25 μmol/L (P<0.05). Conclusion:The antioxidant and antimicrobial properties were enhanced by the complexation of rosmarinic acid and zinc ions, which promoted osteoclast proliferation.

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  • 收稿日期:2023-09-07
  • 最后修改日期:2023-11-14
  • 录用日期:2024-02-27
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