Abstract: Objective: This study aimed to investigate the physicochemical properties of a three-dimensional composite scaffold loaded with curculigoside (CUR) and assess its potential impact on promoting angiogenesis and osteogenic induction in human umbilical vein endothelial cells (HUVECs) and mouse bone marrow mesenchymal stem cells (BMSCs). Methods: ①polycaprolactone microspheres loaded with Curculigoside (CUR-PMs) were prepared using an emulsion/solvent evaporation technique. Subsequently, we successfully engineered a three-dimensional composite scaffold comprising Hydroxyapatite (HA), gelatin (GEL), and Sodium alginate (SA) with the assistance of 3D bioprinting technology, denoted as HGS. Furthermore, we established a polycaprolactone-based microsphere scaffold, referred to as HGSC, for the purpose of Curculigoside loading. The scaffolds underwent thorough characterization through scanning electron microscopy, infrared spectroscopy, rheological analysis, mechanical property assessments, drug release studies, and degradation assessments. ②To confirm the biocompatibility and assess the potential for vascularization and osteogenic regulation of the scaffold, a series of experiments were conducted. These included the CCK-8 assay, EdU fluorescent staining, alkaline phosphatase (ALP) staining, and HUVECs cell tube formation assay. Results: ①Inside the microsphere composite scaffold, there was a consistent grid-like structure of uniform size. When compared to the scaffold without loaded microspheres (HGS), it demonstrated suitable mechanical performance and degradation properties.②The findings from CCK-8 and EdU fluorescence staining demonstrated the excellent biocompatibility of the HGSC scaffold.③The outcomes of HUVECs tube formation experiments and ALP staining of BMSCs provided evidence that the HGSC scaffold exhibited the capability to enhance both bone formation and angiogenesis. Conclusion: The HGSC composite scaffold has shown excellent biocompatibility and has a clear promoting effect on the osteogenic potential in BMSCs and angiogenesis in HUVECs. This conclusion can provide a new example of combining traditional herbal medicine with bone tissue engineering, offering a promising treatment strategy for the repair of bone defects.