Objective: To obtain Dabie bandavirus (DBV) specific antibody sequences from peripheral blood mononuclear cell (PBMC) of recovery patients with severe fever with thrombocytopenia syndrome (SFTS). Then prepared fully human antibodies against DBV. Methods: Enzyme-linked Immunosorbent Assay (ELISA) was used to detect the plasma antibody of SFTS recovery patients. PBMC samples from the recovery patients were selected for magnetic bead enrichment and flow cytometry to obtain the specific single B cells with DBV glycoprotein (DBV-Gn) protein. Performed prokaryotic expression of the single chain antibodies using the antibody sequences obtained from B-cell receptor (BCR) sequencing. Finally, the expression, the binding capacity and neutralizing activity of the antibodies were identified by Western blot, ELISA and neutralization experiment in vitro. Results: 25 blood samples from SFTS recovery patients were collected. The antibodies of the plasma showed binding activity with DBV-Gn and nucleocapsid protein (DBV-NP). Six types of single chain antibodies were obtained after flow cytometry and BCR sequencing analysis, all of which had good binding capacity and neutralizing activity with DBV. Conclusion: Six fully human antibodies with neutralizing activity to DBV have been successfully expressed.