In this experiment, we investigated the frequency of anovulation in a sample of 38 healthy women by collection two to three consecutive menstrual cycles. Methods Daily morning first urine samples were collected for analysis of urine progesterone (pregnanediol 3 glucttronide), FSH and creatinine by enzyme immunoassay. Results Frequency of anovulatory women was 7. 90%, and anovulatory menstrual cycles frequency was 7. 69%. During early follicular phase, FSH excretion was significantly less in the anovulatory cycles than ovulatory cycles, and progesterone always remained at very low level for the whole cycle. Conclusion Urine progesterone of the enzyme immunoassay can be used to evaluate ovary function and to analyze anovulatory menstrual cycle.