Different aprotinin regimens and their hemostatic effects were comparatively analysed and the more appropriate aprotinin regimen was evaluated and discussed here. Methods Eighty-two open heart patients were divided into three groups. There were high-dose half-dose and low-dose aprotinin groups. Results It was shown that the high-dose aprotinin regimen could reduce postoperative drainage by 54%and 55%during the first 12 and 24 hours,meanwhile the hemoglobin loss and blood transfusion requirement were reduced by 56%and 65%respectively compared to the control group. The half-dose aprotinin regimen could reduce postoperative drainage by 49%and 55%in the first 12 and 24 hours ,which was not significantly different as compared with high-dose aprotinin group. The low-dose aprotinin regimen could reduce tube drainage and blood transfusion requirement by 30%and 26%, which had significant difference as compared to half dose aprotinin group. Conclusion The use of aprotinin could sig nificantly reduce postoperative drainage and blood transfusion requirement,the hemostatic effect was related to apro tinin dose. The half-dose and low-dose aprotinin regimen are recommended.