Ｏbjective：To construct plEGFP-N1 mammalian expression vector system containing human full-length HGF cDNA, and to study the packaging efficiency of phoenix cells and the expression of HGF in bone marrow stem cells（MSCs）. Methods：The plasmid plEGFP-N1 and expression plasmid pCMV-HGF were cut with BamHI enzyme respectively. HGF cDNA was obtained and subcloned into plEGFP-N1 expression vector. The recombinant plEGFP-HGF was identificated with restriction enzyme digestion and sequencing. Subsequently, plEGFP-HGF was transformed into phoenix cells and the supernatant was collected. The expression of HGF in the transfected cells was detected by ELISA. RT-PCR was used to determine the expression the receptor of HGF, c-met mRNA in plEGFP-HGF+MSCs. Results：The mammalian expression vector system plEGFP-HGF was digested with BamHI and HindⅢ. Agarose gel electrophoresis showed that there were fragments of 2.3 kb（HGF） and 6.9 kb（plEGFP-N1）. DNA sequencing of HGF was identical to the report in Genebank. The results of RT-PCR showed that c-met transcript strongly increased in pEGFP-HGF／MSCs, suggesting that HGF amplified its own action via stimulation of c-met expression. Conclusion：The plEGFP-HGF mammalian expression vector system containing human full-length HGF cDNA could be constructed successfully, and they could be effectively expressed in MSCs.