bFGF对成骨细胞整合素β1亚单位表达和细胞黏附的影响
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国家高技术研究发展计划(863)资助项目(2002AA205011);南京医科大学科技创新基金(CX2003016);南京医科大学第一附属医院人才引进基金


Effects of basic fibroblast growth factor(bFGF) on expression of β1 integrin subunit and adhesion of osteoblast
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    摘要:

    目的:研究碱性成纤维细胞生长因子(bFGF)对成骨细胞整合素β1亚单位表达的影响,探讨纤维黏连蛋白(FN)与碱性成纤维细胞生长因子(bFGF)单独或联合应用时对成骨细胞在生物衍生骨支架上黏附的影响。方法:培养成骨细胞,以bFGF(100 ng/ml)刺激,接种于FN(10 μg/ml)修饰的生物衍生骨支架上,Western blot检测bFGF对FN受体整合素β1亚单位表达的影响, MTT检测组间细胞黏附效率差异,电镜观察细胞密度与形态。结果:Western blot检测发现应用bFGF刺激后,成骨细胞整合素β1亚单位表达增强;MTT检测发现单独应用FN与bFGF均可增加细胞在生物衍生骨上的黏附效率(P < 0.01),但二者联合应用时,产生的效应进一步增强,二者存在统计学上的交互作用(P < 0.01);电镜观察也证明,二者联合应用时细胞密度、铺展效果均优于单独应用。结论:bFGF可以显著增强成骨细胞FN受体整合素β1亚单位的表达,明显提高成骨细胞在FN修饰生物衍生骨支架上的黏附效率,bFGF与FN对于促进成骨细胞的黏附具有交互作用。

    Abstract:

    Objective:To investigate the effects of basic fibroblast growth factor(bFGF) on the expression of β1 subunit of integrin and to explore the effects of bFGF and fibronection(FN) on osteoblast adhesion on bio-derived bone. Methods:The third generation of osteoblast was treated with bFGF(100 ng/ml) and then seeded on bio-derived bone which had been modified with FN(10 μg/ml). Expression of β1 subunit of integrin was detected by Western blot. Cell adhesion was measured by MTT assay. Cell density and appearance was observed by scanning electron microscope. Results:The expression of β1 subunit of integrin was significantly enhanced by bFGF. Both FN and bFGF could enhance osteoblast’s adhesion efficiency on bio-derived bone(P < 0.01). But the osteoblast’s adhesion efficiency was significantly strengthened by combined use of FN and bFGF. They had a significant synergistic effect in statistics(P < 0.01). Combined use of FN and bFGF had a better effect on cell density and appearance than solo use when observed with scanning electron microscope. Conclusion:BFGF can significantly enhance the expression of β1 subunit of integrin and significantly strengthen osteoblast’s adhesion efficiency on bio-derived bone which has been modified with FN. BFGF and FN have a significant synergistic effect on osteoblast’s adhesion.

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冯 立,曹晓建,任永信,杨志明. bFGF对成骨细胞整合素β1亚单位表达和细胞黏附的影响[J].南京医科大学学报(自然科学版),2007,(1):1-5

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  • 收稿日期:2006-08-09
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