Objective：To investigate the effects of basic fibroblast growth factor（bFGF） on the expression of β1 subunit of integrin and to explore the effects of bFGF and fibronection（FN） on osteoblast adhesion on bio-derived bone. Methods：The third generation of osteoblast was treated with bFGF（100 ng／ml） and then seeded on bio-derived bone which had been modified with FN（10 μg／ml）. Expression of β1 subunit of integrin was detected by Western blot. Cell adhesion was measured by MTT assay. Cell density and appearance was observed by scanning electron microscope. Results：The expression of β1 subunit of integrin was significantly enhanced by bFGF. Both FN and bFGF could enhance osteoblast’s adhesion efficiency on bio-derived bone（P < 0.01）. But the osteoblast’s adhesion efficiency was significantly strengthened by combined use of FN and bFGF. They had a significant synergistic effect in statistics（P < 0.01）. Combined use of FN and bFGF had a better effect on cell density and appearance than solo use when observed with scanning electron microscope. Conclusion：BFGF can significantly enhance the expression of β1 subunit of integrin and significantly strengthen osteoblast’s adhesion efficiency on bio-derived bone which has been modified with FN. BFGF and FN have a significant synergistic effect on osteoblast’s adhesion.