反相高效液相色谱法测定兔房水中万古霉素含量
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R969.1

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Determination of vancomycin levels in rabbit aqueous humor by reverse-phase high performance liquid chromatography
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    目的:应用反相高效液相色谱(RP-HPLC)技术,建立定量检测兔房水万古霉素的方法。方法:房水样品中加入适量10%硫酸锌,高速离心,蛋白质沉淀后进行RP-HPLC检测。检测条件:分析柱为C8柱;流动相为甲醇(A)-醋酸(B),梯度洗脱。兔眼结膜囊内滴入纳米-万古霉素滴眼液后于120 min收集房水样本检测万古霉素浓度。结果:房水中药物峰与杂质峰分离良好,在0.5~20.0 μg/ml范围内,峰面积与浓度呈良好的线性关系。1、10、18 μg/ml 3种浓度的平均回收率为(98.670 ± 0.321)%,日内和日间RSD分别为(3.40 ± 1.30)%,(3.80 ± 2.20)%。兔眼房水中万古霉素药物浓度在滴入纳米-万古霉素滴眼液后120 min为:0.83 ± 0.05 μg/ml。结论: 建立了简便、敏感的检测房水中万古霉素的RP-HPLC方法,可用于眼内万古霉素药代动力学研究。

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    Objective:To establish a reverse-phase high performance liquid chromatographic(RP-HPLC) assay for the determination of vacomycin levels in rabbits aqueous humor. Methods:The vancomycin extracted by the sedimentation with 10% ZnSO4 was detected through RP-HPLC. The analytical column was a C8 column. Twenty microlitres of samples were injected directly into the column and were eluted with a gradient consisting of methanol(A) and acetic acid(B). The sample was collected and analyzed after nano-vancomycin eyedrops administered. Results:The drug peak was separated well without interference from the aqueous humor. The peak area and concentration of the drug in aqueous humor were linearly correlated within the range of 0.5-20 μg/ml, with a correlation coefficient of 0.9990. The average recovery rate of the samples of 1,10,18 μg/ml was(98.670 ± 0.321)%, and the precision and accuracy of the inter- and intra-day was(3.40 ± 1.30)%,(3.80 ± 2.20)% respectively. The concentration of vancomycin in rabbits aqueous humor at 120 min after nano-vancomycin eye drops administered was 0.83 ± 0.05 μg/ml. Conclusion:RP-HPLC assay is simple and sensitive, which may be used in ocular pharmacokinetic studies of vancomycin.

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王 淼,赵人琤,河 清.反相高效液相色谱法测定兔房水中万古霉素含量[J].南京医科大学学报(自然科学版),2007,(1):27-30

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  • 收稿日期:2006-07-12
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  • 在线发布日期: 2007-01-15
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