哮喘小鼠气道平滑肌细胞增殖及细胞周期蛋白D1表达的差异
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Difference of cell proliferation and the expression of cyclin D1 in the airway smooth muscle cells of murine asthmatic model
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    摘要:

    目的:研究哮喘小鼠气道重建模型中气道平滑肌细胞(ASMC)的细胞增殖、细胞周期及细胞周期蛋白D1(cyclin D1)表达的变化,探讨cyclin D1对支气管哮喘气道重建中平滑肌细胞增殖过程及细胞周期的影响,进而为哮喘的诊断和治疗提供新的依据。方法:用Balb/c小鼠建立哮喘气道重建模型后进行ASMC原代培养,并以原代培养的正常小鼠ASMC为对照组,以四唑盐比色试验(MTT)检测细胞增殖能力,流式细胞术(FCM)测定细胞周期,FCM检测cyclin D1表达。结果:与对照组比较,哮喘组细胞的细胞增殖显著增快(P < 0.05);细胞周期中S 期比例明显增高;cyclin D1在胞质中表达明显增加。结论:在哮喘气道重建过程中ASMC经历了一定程度的过增殖过程,cyclin D1的表达增加参与了细胞增殖,气道重建环境可刺激气道平滑肌细胞增殖能力。

    Abstract:

    Objective:To investigate the cell proliferation and cell cycle and the expression of cyclin D1 in the primary cultred airway smooth muscle cells(ASMC) in murine model of asthma. Methods:The murine asthmatic model was established and the primary ASMC were cultured. The primary ASMC of normal mice were chosen as control group. The cell proliferation was detected by MTT. The cell cycle was measured by FCM, and the expression of cyclin D1 was measured by FCM. Results:Compared with the normal ASMC line,the cell proliferation and the percentage of S phase of asthmatic ASMC line was significantly increased(P < 0.05). The expression of cyclin D1 was significantly increased compared with the normal cell line. Conclusion:The proliferation of ASMC is increased in the progression of airway remodeling in asthma and cyclin D1 may contribute to this progression.

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卓致远,黄 茂,葛海燕,王正东,崔学范,殷凯生.哮喘小鼠气道平滑肌细胞增殖及细胞周期蛋白D1表达的差异[J].南京医科大学学报(自然科学版),2007,(1):43-46

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  • 收稿日期:2006-07-11
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