Objective：To investigate the effects of canthaxanthin on the anti-oxidative damage function of osteoblast. Methods： M3T3-E1 osteoblasts were randomly divided into five groups, including control group，model group，canthaxanthin groups［low-dose（1×10-8 mol／L），middle-dose（1 × 10-7 mol／L），high-dose（1 × 10-6 mol／L）］. The activity of cells, superoxide dismutase（SOD）, the content of reactive oxygen species（ROS）, lipid oxygen（LPO） and membrane fluidity were tested and compared. Results：Compared with canthaxanthin groups，the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased（P﹤0.01）. However, the content of ROS and LPO were significantly increased（P﹤0.01）. Conclusion：H2O2 can induce oxidative damage of M3T3-E1 osteoblasts, but canthaxanthin can prevent and decrease its influence.