GFPu-1克隆细胞反映泛素蛋白酶体功能
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Establishment of GFPu-1 clonal cells as an indicator of ubiquitin-proteasome system
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:建立反映泛素蛋白酶体系统(ubiquitin-proteasome system,UPS)功能的GFPu-1细胞系。方法: 将GFPu质粒,转染HEK293细胞,通过G418筛选培养出含GFPu的克隆细胞系GPPu-1,加入蛋白酶抑制剂 lactacystin,通过共聚焦显微镜和Western 印迹观察绿色荧光蛋白(GFP)的表达和分布。结果:加入lactacystin 后,GFPu转染细胞荧光强度明显增强,GFPu在胞浆和细胞核分布均匀,且GFPu和β-catenin明显积聚,GFPu增加了约2.5倍(P < 0.05),β-catenin增加了约1.2倍(P < 0.05)。结论:GFPu-1克隆细胞是一种反映UPS功能的敏感指标。

    Abstract:

    Objective:To establish GFPu-1 clonal cells reflecting the function of the ubiquitin-proteasome system. Methods:GFPu plasmid was transfected into human embryonic kidney(HEK) 293 cells. GFPu-1 cells with stable expression of GFPu were selected by G418. After proteasome inhibitor lactacystin was added into GFPu-1 cells, the distribution and expression of green fluorescent protein(GFP) were studied by confocal microscopy and Western blot. Results:GFPu was distributed diffusely in the nuclear and cytoplasmic compartment of GFPu-1 cells with lactacystin. Compared with the control cells with DSMO, the fluorescence of GFPu significantly increased in GFPu-1 cells with lactacystin. Accummuation of GFPu rose about 2.5 folds(P < 0.05) and β-catenin expression rose about 1.2 fold(P < 0.05). Conclusion:GFPu-1 clonal cells may be a sensitive indicator for the function of the ubiquitin-proteasome system.

    参考文献
    相似文献
    引证文献
引用本文

黄 为,徐 标,Wang Xuejun. GFPu-1克隆细胞反映泛素蛋白酶体功能[J].南京医科大学学报(自然科学版),2007,(2):126-128

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2006-08-11
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明