Objective：To investigate the effect of prostaglandin E2 on the proliferation of human cholangicarcinoma cell and the possible mechanism. Methods：Human cholangicarcinoma cell line（HUCCT1） was cultured and treated with PGE2, and the cell growth rate and viability was measured by cell proliferation assay with WST-1 regent. The intracellular free calcium concentration was measured using laser scanning confocal microscopy with fluo-3／AM as fluorescence probe. The level of cAMP was measured by immunoassay KIT. Results：PGE2 promoted the growth of cholangicarcinoma cell, and increased intracellular free calcium and cAMP in cholangicarcinoma cell. Conclusion：The results that PGE2 could increase the concentration of intracellular free calcium and cAMP suggest that PGE2 may play a role in promoting the cell growth.