曲格列酮抑制甲状腺乳头状癌细胞生长的体外试验
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江苏省教育厅高校自然科学研究计划资助项目(02KB320004)


Inhibitory effects of troglitazone on proliferation of papillary thyroid carcinoma cell
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    摘要:

    目的: 研究曲格列酮对甲状腺癌细胞IHH-4生长的影响及其作用机制。方法:将甲状腺癌细胞IHH-4与不同浓度的曲格列酮共培养,4-甲基偶氮四唑蓝(MTT)法观察其对甲状腺癌细胞增殖的影响,流式细胞仪DNA定量法分析曲格列酮对甲状腺癌细胞细胞周期的影响,半定量RT-PCR法测定曲格列酮对甲状腺癌细胞p27 mRNA 表达的影响。结果: 曲格列酮可以抑制甲状腺乳头状癌细胞的增殖,并具有时间和浓度依赖性(P < 0.05);曲格列酮作用后,甲状腺癌细胞IHH-4的G0/G1细胞的比例增加(P < 0.05);p27 mRNA 的表达显著升高(P < 0.05)。结论: 曲格列酮可以抑制甲状腺癌细胞从G1期向S期的转化,从而抑制其增殖,曲格列酮可能成为治疗难治性甲状腺癌的一种选择。

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    Objective:To investigate the effects of troglitazone on growth of thyroid papillary carcinoma cell line IHH-4 and the mechanisms possibly involved. Methods:Papillary thyroid carcinoma cell line IHH-4 was incubated with different concentrations of troglitazone and observed at different times, and the cell viabilities were measured through dimethylthiazol-diphenyltetrazolium bromide(MTT) assay. The cell cycles of the stimulated cells were analyzed by flow cytometer, and the expression of p27 mRNA was evaluated by semi-quantitative RT-PCR. Results:Troglitazone inhibited the proliferation of thyroid carcinoma cells in a dose-dependent and time-dependent manner(P < 0.05). After treated by troglitazone,the proportion of cells in GO/G1 phase increased significantly(P < 0.05), and p27 mRNA expression was up-regulated. Conclusion:Troglitazone inhibits the growth of papillary thyroid carcinoma cells by arresting the G1/S phase transition, so troglitazone could be a choice for the treatment of the aggressive thyroid carcinoma.

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侯大卫,沈美萍,刘翠萍.曲格列酮抑制甲状腺乳头状癌细胞生长的体外试验[J].南京医科大学学报(自然科学版),2007,(3):249-252

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  • 收稿日期:2006-10-08
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