Objective：To investigate the effects of epidermal growth factor（EGF） on endothelin-1（ET-1） secretion and mRNA expression in hormone refractory prostate cancer（HRPC） PC-3 cell line. Methods：The expression of ET-1 mRNA was detected by RT-PCR and ET-1 secretion was detected by radioimmunoassay（RIA） in PC-3 cell line treated with EGF. The change of ET-1 secretion after the treatment with different doses of EGF was measured by RIA. Results：After the treatment with EGF, the expression of ET-1 mRNA and ET-1 secretion in PC-3 cell line was up-regulated, and there was significant difference（P ＜ 0.05） between experimental group and control group. After the treatment with different doses of EGF, ET-1 secretion was up-regulated as the dose of EGF increased. One-factor analysis of variance indicated the effects of different doses of EGF on secretory volume of ET-1 were different（P ＜ 0.05）. Conclusion：The expression of ET-1 mRNA and ET-1 secretion in PC-3 cell line is up-regulated by EGF which plays a role in the progress of prostate cancer.