99mTc标记抗心肌肌钙蛋白Ⅰ单克隆抗体在实验性急性心肌损伤大鼠体内分布的研究
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Distribution of 99mTc-AcTnIMA in rats with acute myocardial injury
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    目的:研究99mTc标记的抗心肌肌钙蛋白Ⅰ单克隆抗体(AcTnIMA)在实验性急性心肌损伤大鼠体内的分布,探讨99mTc-AcTnIMA是否可以作为心脏放射免疫显像剂。方法:实验组:20只急性心肌损伤大鼠注射99mTc-AcTnIMA 0.2mci,分别于注射后2、4、6、8 h处死(每次5只),取血液、肝、脾、肾、正常肌肉、肺和心脏,以计算每克组织放射性计数占总注入计数的百分比(ID%/g)及心-肺ID%/g比(HLR)。药物对照组:20只急性心肌损伤大鼠注射99mTc标记的非特异性免疫球蛋白(N-IgG)0.2mci,处死方法同实验组。取肺及心脏,计算ID%/g及HLR;空白对照组:20只正常大鼠注射99mTc-AcTnIMA 0.2mci,处理方法同药物对照组。结果:急性损伤心肌对99mTc-AcTnIMA为特异性摄取,摄取的高峰时间为4 h。结论:99mTc-AcTnIMA有望作为心脏放射免疫显像剂诊断急性心肌损伤。

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    Objective:To investigate the distribution pattern of 99mTc-AcTnIMA in rats with experimental acute myocardial injury, and to determine the possibility of 99mTc-AcTnIMA to be a tracer of myocardial radioimmunoimaging for the detection of myocardial injury. Methods:In the experimental group,20 rats with acute myocardial lesion were injected with 0.2mci 99mTc-AcTnIMA, and killed 2, 4, 6 h and 8 h after injection respectively(5 rats). Blood, liver, spleen, kidney, healthy muscle, lung and heart muscle of each rat were taken and the injected dosage(ID%/g) and the ratio of ID%/g for heart to lung(HLR) was calculated. In the pharmic control group,20 rats with acute myocardial lesion were injected with 0.2mci 99mTc-N-IgG and were killed in the same way as the experimental group. Lungs and hearts were taken and ID%/g and HLR of each rat was calculated. In the blank control, 20 healthy rats were injected with 0.2mci 99mTc-AcTnIMA and the subsequent procedures were same as the pharmic group. Results:Specific uptake of 99mTc-AcTnIMA by acute compromised cardiac muscles was observed with its peak 4 h after injection. Conclusion:99mTc-AcTnIMA could be a useful tracer of myocardial radioimmunoimaging to diagnose acute myocardial injury.

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李建华,王德杭,李殿富,徐兆强,张寄南.99mTc标记抗心肌肌钙蛋白Ⅰ单克隆抗体在实验性急性心肌损伤大鼠体内分布的研究[J].南京医科大学学报(自然科学版),2007,(4):385-388

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  • 收稿日期:2006-05-24
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