Objective：To explore the effect of human growth hormone（GH） on bile duct cancer cell line（QBC939 cell） and pancreatic carcinoma cell line（BXPC-3 cell） in vitro and the possible mechanism. Methods：The QBC939 and BXPC-3 cells during exponential growth stage were harvested and divided into experimental group and control group. The experimental group was separated into four sub-groups（50 μg／L 2 h，50 μg／L 24 h，100 μg／L 2 h，100 μg／L 24 h） according to the dose of GH and culture time. After 2 h and 24 h, Insulin-like Growth Factor1 was detected by using ELISA respectively. The carcinoma cells cultured for 24 h with various GH concentration（50 μg／L，100 μg／L） were made into single cell suspentions, and the samples underwent subsequent cell cycle evaluation. IGF1RmRNA was tested with the method of hybridization in vitro. Results：GH stimulated cell growth in vitro and also elevated S phase percent and proliferation index（PI） of cells after 24 h culture（P < 0.05）. The quantum of IGF1 was significantly increased after 2 h culture in GH groups, but there was no statistical significance between GH groups and NS groups 24 h ago. IGF1RmRNA was expressed in two carcinoma cell lines in contrast to blank controls, and the expression boosted up according to the dose of GH. Conclusion：GH stimulates QBC939 and BXPC-3 cell proliferation in vitro，and the possible mechanism is acted by the axis of GH-IGF1.