他莫昔芬对单侧输尿管梗阻大鼠肾间质纤维化的保护作用
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

江苏省教育厅资助项目(03KJD320125)


Effect of Tamoxifen on interstitial fibrosis in UUO rats
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨他莫昔芬对肾组织纤维化的影响及作用机制。方法:雌性SD大鼠,建立单侧输尿管梗阻模型(UUO),设:①模型组(n = 6),行左侧输尿管结扎术,0.3 ml无菌生理盐水灌胃,每天2次;②治疗组(n = 6),他莫昔芬5 mg/kg(0.3 ml无菌生理盐水配置) 灌胃,每天2次;③假手术组(n = 6)。术后第9天处死各组大鼠。采用光镜和免疫组化观察肾组织纤维化和α平滑肌肌动蛋白(α-SMA)表达的变化。结果:① 治疗组大鼠肾间质纤维化程度较模型组明显减轻。② UUO模型第9天时大鼠肾组织α-SMA表达明显增强,采用他莫昔芬治疗后,肾组织α-SMA表达的异常增强能得到有效抑制。结论:昔芬类药物具有减轻肾组织纤维化的作用,而这一作用与其能有效阻抑肾组织上皮细胞-间充质细胞转分化(EMT)有关。

    Abstract:

    Objective:To investigate the effects of Tamoxifen on renal interstitial fibrosis in UUO rats. Methods:Feamale SD rats were divided into 3 groups at random:①UUO group(6 rats), ②the treated group(6 rats):UUO + Tamoxifen 10 kg/(mg·d) day by gastrogavage, ③sham operated group(6 rats). Kidney were harvested at day 9 after UUO. The renal tissues were examined by HE,PAS and Masson. Tissue α-SMA protein expression was determined by Immunohistochemistry. Results:UUO for 9 days significantly activated α-SMA protein expression, induced interstitial fibrosis and tubulointerstitial lesion in obstructed kidney of UUO group, compared with sham operated group rats. Conversely daily administration of Tamoxifen significantly inhibited α-SMA expression and decreased interstitial fibrosis and tubulointerstitial lesion in obstructed kidney of treated group. Conclusion:Tamoxifen had beneficial effects on reduction of renal interstitial fibrosis in SD rats with UUO, and this effect may be associated with Tamoxifen inhibits α-SMA expression.

    参考文献
    相似文献
    引证文献
引用本文

姚刚,许宏岳,杨春,吴限,徐玲玲.他莫昔芬对单侧输尿管梗阻大鼠肾间质纤维化的保护作用[J].南京医科大学学报(自然科学版),2007,(5):480-482

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2006-11-16
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明