木犀草素对哮喘气道重塑小鼠模型气道IL-13Rα2表达的影响
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Effect of luteolin on expression of IL-13Rα2 in the airway of asthmatic mice with airway remodeling
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:观察木犀草素对哮喘小鼠气道重塑和气道IL-13Rα2表达的影响。方法:建立哮喘气道重塑模型,自第14 天雾化吸入OVA 前0.5 h,干预组分别腹腔注射地塞米松及木犀草素灌胃。OVA 雾化结束后24 h,处死小鼠后肺组织石蜡切片HE染色观察气道形态学改变,IL-13Rα2免疫组化染色,并经计算机图像分析测定气道壁中含量,同时RT-PCR法测定IL-13Rα2、TGF-β1mRNA的表达。结果:①光镜下模型小鼠均有气道重塑病理改变,哮喘组明显,地塞米松干预组和木犀草素干预组较轻;②气道图像分析哮喘组支气管平滑肌的面积/气管腔的内周长(Wam/Pi)值、Wam/Pi值较空白对照组显著增加,地塞米松组和木犀草素干预组较哮喘组显著减轻;③免疫组化分析单位长度基膜气道壁平均IL-13Rα2阳性细胞数,哮喘组小鼠较正常组升高,地塞米松干预组和木犀草素干预组较哮喘组明显减少;④RT-PCR检测哮喘组小鼠气道壁IL-13Rα2及TGF-β1含量较正常组显著增加,地塞米松干预组和木犀草素干预组较哮喘组明显减少。结论:木犀草素可抑制气道壁的增厚和平滑肌增殖,减轻气道重塑,其作用的机制可能是通过抑制IL-13Rα2的表达实现的。

    Abstract:

    Objective:To investigate the effects of luteolin on airway remodeling and the expression of IL-13Rα2 in the airway of chronicity asthmatic mice. Methods:Thirty-two BALB/c mices were randomly divided into four groups. The mouse asthma models(asthma group) were established by sensitization and challenge with ovalbumin(OVA), eight mices interfered with luteolin 30 min before challenge(luteolin group), eight mices administrated dexamethasone by intraperitoneal injection 30 min before challenge(dexamethasone group). The healthy control mice were treated with saline instead of OVA. Lung tissu were collected 24 h after the last challenge. The left lung tissue was enbedded in paraffin and sectioned. Henatoxylin and esin(HE) staining were performed,Histologocial changes were detected. The expressions of genes and their prouducts of IL-13Rα2 in airway were detected using RT-PCR and immunohistochemistry(ISH). The expressions of genes of TGF-β in lung tissue were detected using RT-PCR. Results:①HE-stained lung tissue showed there were airway remodeling in each group,the thickness of airway in luteolin group was significantly decreased compared with asthma group and increased compared with normal group.②Wat/Pi and Wam/Pi in luteolin group and dexamethasone group were significantly lower than those in asthma group.③RT-PCR showed that expression of IL-13R2mRNA and TGF-β1mRNA in luteolin group and dexamethasone group were significantly lower than those in asthma group. ④ISH Showed that expression of IL-13Rα2 in luteolin group and dexamethasone group were significantly lower than those in asthma group. Conclusion:Luteolin might inhibit the thicking of airway wall and airway smooth muscle proliferation, and inhibit airway remodeling. Its possible mechanisms might be of inhibiting the expression of IL-13Rα2 in the lung tissue.

    参考文献
    相似文献
    引证文献
引用本文

王正东,黄 茂,范文辉,卓致远,殷凯生.木犀草素对哮喘气道重塑小鼠模型气道IL-13Rα2表达的影响[J].南京医科大学学报(自然科学版),2007,(5):491-494

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2006-10-23
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明