大鼠妊娠期胰岛形态及β细胞增殖能力的变化
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

江苏省医学重点学科资助项目(SK200214)


Adaptation on the morphology and proliferation of pancreatic islet during pregnancy in rats
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨大鼠妊娠不同阶段胰岛形态及β细胞增殖的变化-方法:采用5-溴尿脱氧嘧啶核苷(BrdU)及抗胰岛素抗体免疫组化双染法,通过形态学及量化分析,研究大鼠妊娠10.5-12.5-14.5-18.5和20.5天时胰岛形态的改变及β细胞的增殖率的改变-结果:与正常对照组相比, 大鼠妊娠12.5天时胰岛素染色阳性区域平均光密度-胰岛素相对浓度(IRC)-胰岛素阳性表达细胞密度(PCD)以及胰岛β细胞增殖指数(PI)均明显增高,并于孕14.5天到达高峰,而后逐渐下降至对照组水平-此外,大鼠妊娠14.5天至20.5天时胰岛素阳性表达细胞面积亦明显高于正常水平-结论:大鼠妊娠期胰岛β细胞的增殖能力和胰岛素表达,均从孕12.5天开始发生改变,孕14.5天变化最为显著,并持续至孕晚期(孕20.5天)-

    Abstract:

    Objective:To elucidate the adaptive changes on the morphology and proliferation of pancreatic islet during pregnancy in rats. Methods:Rat pancreatic tissues of each group were double-stained by 5-bromodeoxyuridine(BrdU) and anti-insulin antibody by immunohistochemistry technique to measure the changes of islet morphology and quantity during pregnancy at day 10.5,12.5, 14.5,18.5 and 20.5. Results:Compared with the normal control group, the average photodensity of insulin-expression positive zone, insulin relative concentration(IRC), insulin-expression positive cell density(PCD) and proliferation index(PI)of pancreatic islet were all significantly increased on day 12.5, rose continuously to a peak at day 14.5 of pregnancy, and then gradually returned to levels as control. In addition, areas of insulin-expression positive cells were also much greater during pregnancy during day 14.5 to day 20.5 than in non-pregnant rats. Conclusion:Both pancreatic islet -茁 cell proliferation and insulin-expression concentration became more significantly increased than sex-matched virgin control rats from day 12.5, peaked at day 14.5 of pregnancy,and lasted to late pregnancy(day 20.5).

    参考文献
    相似文献
    引证文献
引用本文

薛 莹,刘翠萍,袁庆新,徐宽枫,刘 超.大鼠妊娠期胰岛形态及β细胞增殖能力的变化[J].南京医科大学学报(自然科学版),2007,(7):698-701

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2007-01-01
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明