川芎嗪对哮喘小鼠干细胞因子的抑制作用
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Effect of ligustrazine inhibition on the expression of stem cell factor(SCF) in mouse asth-matic models
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    摘要:

    目的:探讨川芎嗪对哮喘小鼠血清干细胞因子(stem cell factor,SCF)表达的影响,及抑制气道炎症的作用-方法:24只雄性BALB/c小鼠按随机数字表法分成正常对照组(A组)-哮喘模型组(B组)-小剂量川芎嗪组(C组,40 mg/kg)-大剂量川芎嗪组(D组,80 mg/kg),每组6只-用苏木精-伊红染色观察气道病理改变;用ELISA法检测小鼠血清中SCF含量-结果:小鼠血清中SCF含量哮喘组(114.9 ± 27.3)pmol/L明显高于正常对照组(48.6 ± 11.2)pmol/L(P < 0.01);小剂量川芎嗪组(70.6 ± 7.9)pmol/L与大剂量川芎嗪组(51.4 ± 8.1)pmol/L 均明显低于哮喘组(P均 < 0.01);肺组织中嗜酸性粒细胞(EOS)和淋巴细胞总数,管壁面积/支气管管腔内周长(WA/Pi)和支气管平滑肌面积/支气管管腔内周长(ASM/Pi)哮喘组分别为(32.6 ± 4.5)个/mm2-(1.196 ± 0.111)mm-(0.292 ± 0.027)mm,正常对照组分别为(6.6 ± 1.2)个/mm2-(0.571 ± 0.057)mm-(0.139 ± 0.014)mm,两组比较差异有统计学意义(P均 < 0.01);小剂量川芎嗪组分别为(26.0 ± 2.5)个/mm2-(0.949 ± 0.105)mm-(0.243 ± 0.027)mm,与哮喘组比较差异有统计学意义(P均 < 0.05);大剂量川芎嗪组分别为(12.1 ± 1.6)个/mm2-(0.875 ± 0.111)mm-(0.178 ± 0.023)mm,与哮喘组比较差异有统计学意义(P均 < 0.05)- 结论:川芎嗪可能通过抑制血清SCF表达抑制哮喘小鼠气道炎症-

    Abstract:

    Objective:To identify the ligustrazine effect on asthmatic mice serum stem cell factor(SCF) expression and its effects on inhibiting bronchi inflammation. Methods:Twenty-four male BALB/c mice were randomly divided into four groups:the normal control group(A), the asthmatic model group(B),the low-dose ligustrazine group(C,40 mg/kg) and large-dose ligustrazine group(D,80 mg/kg). There were 6 mice in each group. The bronchi pathological changes were detected by HE dyeing and the SCF content was examined by ELISA. Results:The content of SCF in mice in asthmatic model group[(114.9 ± 27.3)pmol/L] is higher than that in normal control group[(48.6 ± 11.2)pmol/L](P < 0.01). The SCF contents in low-dose ligustrazine group[(70.6 ± 7.9)pmol/L] and in large-dose ligustrazine group[(51.4 ± 8.1)pmol/L] are both lower than that in the asthmatic model group(P < 0.01). In the asthmatic group,the numbers of eosinophils and lymphocytes,the thicknesses of WA/Pi and ASM/Pi were(32.6 ± 4.5)/mm2,(1.196 ± 0.111)mm,(0.292 ± 0.027)mm,respectively;and those of the normal group were(6.6 ± 1.2)/mm2,(0.571 ± 0.057)mm,(0.139 ± 0.014)mm,respectively;there were significant difference between the two groups(all P < 0.01), The numbers of eosinophils and lymphocytes,the thicknesses of WA/Pi and ASM/Pi of the low-dose ligustrazine group were(26.0 ± 2.5)/mm2,(0.949 ± 0.105)mm,(0.243 ± 0.027)mm, respectively. They were lower than those in the asthmatic group(all P < 0.05). The numbers of eosinophils and lymphocytes,the thicknesses of WA/Pi and ASM/Pi of the large-dose ligustrazine group were(12.1 ± 1.6)/mm2,(0.875 ± 0.111)mm,(0.178 ± 0.023)mm,respectively. They were significantly lower than those in the asthmatic group(all P < 0.01). Conclusion:Ligustrazine may control the mice bronchi inflammation by inhibiting the SCF expression.

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刘洁,殷凯生,卞涛,薛一峰,胡志刚.川芎嗪对哮喘小鼠干细胞因子的抑制作用[J].南京医科大学学报(自然科学版),2007,(7):729-732

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  • 收稿日期:2007-01-08
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