Objective:To investigate the changes of zinc finger protein 207(ZNF207)gene mRNA during the differentiation of P19 cells to cardiac myocytes, and to explore the relationship between ZNF207 gene level and the differentiation stage of cardiac myocytes. Methods:P19 cells were cultured and induced by 0.9%DMSO to differentiate to cardiac myocytes in vitro. Total RNA were extracted from P19 cells during the process of differentiation at various time points:and the levels of ZNF207 mRNA were evaluated by RT-PCR. Results:When P19 cells were induced by DMSO to differentiate to cardiac myocytes, the expression level of ZNF207 gene mRNA was up-regulated and reached the highest level at 10 th day. There was a significant difference between day 2 to day 10(P < 0.05). Conclusion:The expression of ZNF207 gene was up-regulated in the process of the differentiation of P19 cells to cardiac myocytes,which might be involved in the differentiation of cardiac myocytes.