CBP基因在非小细胞肺癌中的表达及其生物学意义
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国家自然科学基金资助项目(30270335)


Reduction of CBP gene expression in NSCLC:expression and correlation with clinicopat-hologic parameters
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    目的:研究CBP(CSK-binding protein)基因表达下降与人肺癌临床病理生理特征的关系,探讨CBP基因表达与肺癌肿瘤细胞发生-侵袭的相关性-方法:对50例非小细胞肺癌(NSCLC)标本(含癌旁组织)和10例肺良性病变组织应用单克隆抗体-免疫组化SP法检测CBP基因的蛋白表达情况-应用RT-PCR方法检测NSCLC中CBP的mRNA表达水平-结果:肺癌肿瘤组织中CBP基因表达水平显著低于癌旁组织和肺良性病变组织(P < 0.05)-肺癌组织中CBP基因表达水平降低与肿瘤分期-淋巴结转移程度存在相关性(P < 0.05),与肺癌组织学类型-细胞分化程度以及患者的性别-年龄无关-结论:CBP基因可能参与了对肺癌肿瘤细胞Src家族成员激酶活性的负反馈调节,其表达下降可能与肺癌的发生-发展过程有关-

    Abstract:

    Objective:The aim of this study was to assess the expression of protein products of CBP(CSK-binding protein) in non-small cell lung cancer(NSCLC), to estimate their possible expression and to correlate the results of immunohistochemical analysis with various clinicopathologic parameters. Methods:The expression of CBP gene was detected by immunohistochemical S-P method in 50 cases of NSCLC tissues,50 para-cancer tissues and 10 cases of lung benign lesion tissues as control. According to immunohistochemical results, the expressions of CBP mRNA were detected by reverse transcriptase polymerase chain reaction(RT-PCR) in corresponding samples. Results:The expression level of CBP gene was found to be significantly lower in NSCLC tissues than in para-cancer tissues and lung benign lesion tissues(P < 0.05). The expression level of CBP gene was closely related to PTNM stages and lymph node involvement in Lung cancer patients(P < 0.05). Conclusion:CBP gene may play a role in the negative feedback regulation of Src-family kinases in non-small cell lung cancer, and reduction of CBP gene expression may be related to the pathogenesis and development of NSCLC.

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周 栋,邹良建,杨 勇. CBP基因在非小细胞肺癌中的表达及其生物学意义[J].南京医科大学学报(自然科学版),2007,(8):797-800863

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  • 收稿日期:2007-01-21
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