Objective:To explore the expression and significance of WWOX and FHIT in colorectal cancer. Methods:The expression of WWOX and FHIT was evaluated by immunohistochemical staining(two-step method) in 75 colorectal carcinoma specimens and 30 normal paraneoplastic colorectal tissues,and their correlation with clinicopathological features was analyzed. Results:The rate of WWOX and FHIT positive expression in tumors was 52% and 54.7%,respectively,which was significantly different from that in normal paraneoplastic colorectal tissues as 93.3% and 97.8%. WWOX and FHIT expression were correlated strongly(P < 0.05). The expression of WWOX and FHIT was closely associated with histological grade,Duke stage and 5-year survival rate. And the expression of wwox and FHIT had no significant correlation with neoplastic site and ages. There was a correlation between WWOX expression and FHIT This correlation was consistent with the susceptibility of common fragile sites to DNA damage. Conclusion:The positive expression of WWOX and FHIT was lower than the normal tissues and closely associated with histological grade,Duke stage,and 5-year survival rate(P < 0.05). WWOX and FHIT expression were correlated strongly(P < 0.05). It is suggested that WWOX and FHIT genes may play an important role in the initiation and development of colorectal cancer. They can become targets of predicting the progression of colorectal cancer.