Objective:To explore the inhibition of different chemotherapeutic drugs to the breast cancer T47D cell line which highly expresses γ-synuclein(SNCG). Methods:T47D with SNCG expression and MCF-7 without SNCG expression cell lines were routinely cultured. MTT method was employed to evaluate the inhibiting roles of 6 chemotherapeutic drugs(DDP,ADM,5-FU,VCR,TAX and CPT-11) to the two cell lines. Moreover, Flow cytometry was enrolled to analyze the cell cycles and the apoptosis of T47D cell lines. Results:The inhibiting roles of ADM,VCR,TAX and CPT-11 to T47D were much lower than that of MCF-7 cell line after 60 h treatment(P < 0.01), while the effects of 5-FU and DDP to T47D cell line were not only the same as to MCF-7, but also much higher than the other 4 drugs(P < 0.01). Moreover, there were significant differences of PI and caspase-3 expressions of T47D cell line between DDP,5-FU groups and control or other 4 groups, respectively(P < 0.01). Conclusion:The inhibiting effects of DDP,5-FU to T47D cell line with high expression of SNCG are much higher than those of ADM,VCR,TAX and CPT-11,which can provide experimental evidence in vitro for the chemotherapy strategy to breast cancer patients.