Objective:To explore the correlation of two functional nucleotid epolymorphisms in the cyclooxygenase-2(COX-2) gene with susceptibitity to gastric cancer in a Chinese population of Yixing city. Methods:Polymerase chain reaction-restricted fragments length polymorphism(PCR-RFLP) and the primer-introduced restriction analysis(PIRA-PCR) assay was used to defect genotype of the COX-2 -1195G/A and 8473T/C polymorphisms, respectively. In a case-control study, 254 gastric cancer cases and 304 cancer-free controls were included in a Chinese population of Yixing city. Results:There was no statistical difference in the genotype frequencies of these two polymorphisms between the cases and controls(P = 0.695 for -1195G/A and P = 0.762 for 8473T/C). Logistic regression analyses showed that both variant genotypes of COX-2 -1195 and COX-2 8473 polymorphisms were not significantly associated with susceptibility to gastric cancer(adjusted OR=1.09,95% CI=0.70-1.67 for SNP -1195 GA+AA and adjusted OR=1.18,95% CI=0.83-1.69 for SNP8473 TC+CC). Conclusion:These findings suggested that these two COX-2 polymorphisms may not be associated with genetic susceptibility to gastric cancer.