子宫平滑肌瘤细胞原代培养及鉴定
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南京医科大学科技发展基金重点项目(2005NYDZD21),南京市医学科技发展项目(YKK06080)


Primary culture and identification of uterine leiomyoma cells
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    摘要:

    目的:建立人子宫平滑肌瘤细胞原代培养方法,为子宫肌瘤发病机制及治疗药物筛选的研究提供理想的细胞株系-方法:采用消化法及组织块贴壁2种方法进行子宫平滑肌瘤细胞培养-倒置显微镜下观察原代培养的细胞,并用免疫组化染色对其进行鉴定-结果:2种方法均可获得大量子宫肌瘤细胞,所培养的细胞在倒置显微镜下呈梭形和典型“峰-谷”样生长,α-Actin免疫组化染色呈强阳性-结论: 采用该2种方法均可成功进行人子宫平滑肌瘤细胞的原代培养,且培养的人子宫平滑肌细胞生长良好,纯度高,可用于子宫肌瘤发病机制及治疗药物筛选的研究-

    Abstract:

    Objective:To explore the method of primary culture of uterine leiomyoma cells,which provided a cell line for studying the pathogenesis of uterine leiomyoma and drug screening. Methods:Human uterine leiomyoma cells were cultivated by digestive method and explanted tissue culture in vitro. The surviving cells were observed by phase contrast microscope and identified by immunohistochemistry staining. Results:Many uterine leiomyoma cells were obtained by the above two methods. The cells appeared in spindle shape and showed typical hill-valley pattern under phase contrast microscope. Immunohistochemistry staining with α-Actin demonstrated these cells were positive. Conclusion:Primary culture of uterine leiomyoma cells could be obtained by these two means,with highly purity,and can serve the further studies of the pathogenesis and drug screening of human uterine leiomyoma.

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徐 青,仇黎丽,徐昌芬,罗 莉,朱利群.子宫平滑肌瘤细胞原代培养及鉴定[J].南京医科大学学报(自然科学版),2007,(9):967-969

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  • 收稿日期:2007-02-20
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