氨基胍对大鼠急性高眼压视网膜一氧化氮合酶分布的影响
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The effect of aminoguanidine on the level of nitric oxide synthase on retina in high ocular pressure rat models
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    摘要:

    目的:观察大鼠急性高眼压模型中视网膜一氧化氮合酶(nitric oxidesynthase,NOS)分布及含量的变化,探讨氨基胍(AG)在高眼压诱导的视神经损伤中的神经保护作用-方法:SD大鼠27只,随机选取3只作为空白对照组(6眼),余24只施行高压前房灌注,眼压升高60 min后,随机取12只腹膜腔内注射氨基胍溶液(实验Ⅱ组),另外12只腹膜腔内注射等量生理盐水(实验Ⅰ组),再分别按高眼压术后时间不同随机分为4组即12 h组-24 h组-3天组和7天组,每组3只6眼;各组应用NADPH-d(还原型尼克酰胺嘌呤二核苷酸磷酸-黄递酶)法检测视网膜NOS的表达及分布情况-结果:空白对照组及实验组各组视网膜均有NOS表达,NOS阳性细胞在视网膜主要分布于视网膜节细胞层(ganglion cell layer, GCL)-内丛状层(inner plexiform layer ,IPL)-内核层(inner nuclear layer,INL); 实验Ⅰ组视网膜GCL的NOS阳性细胞数明显高于空白对照组(P < 0.01),实验Ⅱ组视网膜GCL的NOS阳性细胞数与实验Ⅰ组对照减少差异显著(P < 0.01)-结论:一氧化氮(NO)参与了高眼压诱导的视神经损伤; 氨基胍可能通过对NOS的抑制在大鼠高眼压诱导视网膜损伤中起到视神经保护作用-

    Abstract:

    Objective:To observe the nitric oxide synthase(NOS) expression in retina, and to investigate the function of the NOS inhibitor aminoguanidine(AG) in rats with acute intraocular hypertension. Methods:Twenty-seven SD rats were randomly divided into three groups:blank control(unoperational group),experimental group Ⅰ(ocular hypertension group),experimental group Ⅱ(drug-given group). Then each experimental group was divided into subgroups at 12 hours,24 hours,3 days and 7 days after perfusion,and NOS expression in the retina were detected by NADPH-diaphorase histochemistry. Results:The staining of NOS were localized in retinas of all groups. The count of NOS staining cells in the experimental group Ⅰ was more significant than that in the blank control. After AG was injected, the levels of NOS decreased. Conclusions:NO should play some cytotoxicity roles to retina. By inhibiting the activity of iNOS,AG might protect the retina against acute ocular hypertension injury.

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马艳萍,朱承华,杭 荟.氨基胍对大鼠急性高眼压视网膜一氧化氮合酶分布的影响[J].南京医科大学学报(自然科学版),2007,(9):977-980

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  • 收稿日期:2007-02-10
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