脂多糖对人肾小管上皮细胞株(HK-2)增殖和细胞因子表达的影响
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镇江市社会发展基金(SH2004051)


The expression of inflammatory factor and proliferation of human renal tubular epithelial cell stimulated by LPS
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    摘要:

    目的:观察脂多糖(LPS)对人肾小管上皮细胞株(HK-2)增殖及炎症因子表达的影响。方法:体外培养的人肾小管上皮细胞分为两组:阴性对照组和LPS刺激组(1.0 ng/ml)。应用MTT法检测LPS对HK-2增殖的影响;倒置显微镜观察细胞形态学的变化;间接免疫荧光方法检测白细胞介素-1(IL-1)、白细胞介素-10(IL-10)、肿瘤坏死因子(TNF)的表达;采用ELESA方法检测细胞培养上清中IL-1、IL-10、TNF的含量。结果: LPS组能促进HK-2细胞的增殖,促进细胞表达IL-1、IL-10、TNF。结论:LPS在体外促进HK-2增殖,可分泌促纤维化因子抗纤维化的因子。

    Abstract:

    Objective:To investigate the role of Lipopolysaccharide(LPS) on the proliferation of human renal tubular epithelial cell(HK-2) in vitro. Methods:HK-2 cells cultured were divided into 2 groups, negative control group and LPS treated group(1.0 ng/ml LPS). The effect on the proliferation of HK-2 cells was detected by MTT technique. The morphological changes were observed under an invertedmicroscope. The expressions of interleukin-1(IL-1), interleukin-10(IL-10) and tumor necrosis factor(TNF) were observed by indirect immunofluorescence assay. IL-1, IL-10 and TNF in the cultured supernatant were measured by ELESA. Results:LPS was able to promote the proliferation of HK-2 cells. Conclusion:LPS can promote the proliferation of human renal tubular epithelial cells and expression of inflammatory factor in vitro.

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王美云,陈玉琼,张志坚,端礼荣.脂多糖对人肾小管上皮细胞株(HK-2)增殖和细胞因子表达的影响[J].南京医科大学学报(自然科学版),2007,(11):1261-12631277

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  • 收稿日期:2007-04-19
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