左旋和混旋氨氯地平缩短大鼠心室肌细胞动作电位时程
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江苏省135重点实验室资助项目(RC2003019)


Action potential duration of rat ventricular cardiomycytes could be shorten by amlodipine
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    摘要:

    目的:探讨不同旋体氨氯地平(Aml)对Spraque-Dawley大鼠心室肌细胞动作电位(AP)的影响-方法:采用酶消化法获得大鼠耐钙心室肌细胞,以全细胞膜片钳技术分别记录加入0.1-0.5-1.0-5.0和10.0 -滋mol/L左旋-右旋和混旋Aml后大鼠心室肌细胞AP的变化-结果:① 加入0.1-0.5-1.0-5.0和10.0 -滋mol/L左旋-右旋和混旋Aml后,AP最大上升速率-AP幅度和超射无明显变化(P > 0.05);②加入0.1-0.5-1.0-5.0和10.0 -滋mol/L左旋后,50%的AP时程(APD50)分别为(36.2 ± 8.2)-(33.9 ± 7.7)-(30.2 ± 6.8)-(22.6 ± 5.1)和(15.1 ± 3.4)ms(P < 0.05);加入混旋Aml后,APD50分别为(39.2 ± 9.2)-(36.7 ± 7.9)-(33.8 ± 7.2)-(25.4 ± 5.9)和(21.7 ± 5.2)ms(P < 0.05);加入右旋Aml后,APD50分别为(45.1 ± 11.3)-(46.2 ± 10.8)-(44.9 ± 7.3)-(44.8 ± 8.2)和(45.7 ± 9.4)ms(P > 0.05)-结论:加入左旋和混旋Aml后,随着浓度增加,APD逐渐缩短,左旋和混旋Aml可能是通过阻滞L-型钙离子流影响APD;而右旋Aml对APD无影响,右旋Aml可能对L-型钙离子流无影响-

    Abstract:

    Objective:To investigate effects on action potential(AP) of Spraque-Dawley rat by different isomers of amlodipine(Aml). Methods:Calcium-tolerant ventricular myocytes were isolated by enzyme digestion. AP changes in S-Aml,R-Aml and R,S-Aml with concentrations of 0.1,0.5,1.0,5.0 and 10.0 μmol/L were recorded respectively by whole cell configuration patch clamp. Results:①AP maximal velocity,AP amplitude and overshoot had no significant changes in S-Aml,R-Aml and R,S-Aml with concentrations of 0.1,0.5,1.0,5.0 and 10.0 μmol/L(P > 0.05); ②Fifty percent of Action potential duration(APD50) were(36.2 ± 8.2)-(33.9 ± 7.7)-(30.2 ± 6.8)-(22.6 ± 5.1) and (15.1 ± 3.4)ms,respectively(P < 0.05) with the increase of S-Aml concentrations from 0.1 μmol/L to 10 μmol/L;APD50 were (39.2 ± 9.2),(36.7 ± 7.9),(33.8 ± 7.2),(25.4 ± 5.9) and (21.7 ± 5.2)ms,respectively(P < 0.05) with the increase of P,S-Aml concentrations from 0.1 μmol/L to 10 μmol/L;APD50 were(45.1 ± 11.3),(46.2 ± 10.8),(44.9 ± 7.3),(44.8 ± 8.2) and (45.7 ± 9.4)ms,respectively(P > 0.05) with the increase of P-Aml concentrations from 0.1 μmol/L to 10 μmol/L. Conclusion:APDs were gradually shortened with the increase of S-Aml and R,S-Aml concentrations. S-Aml and R,S-Aml might influence APDs by blocking L-type calcium channel;R-Aml had no effect on APD,and might have no influence on L-type calcium channel.

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来利红,王如兴,蒋文平,刘志华,杨向军,宋建平,蒋 彬,李红霞.左旋和混旋氨氯地平缩短大鼠心室肌细胞动作电位时程[J].南京医科大学学报(自然科学版),2007,(12):1394-1397

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  • 收稿日期:2007-05-08
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