重度子痫前期患者胎盘滋养细胞凋亡现象的研究
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江苏省教育厅自然科学研究基金资助项目(00KJD320012)


Observation on placental trophoblast apoptotic phenomenon in patients with severe pre-eclampsia
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    摘要:

    目的:分析重度子痫前期患者胎盘滋养细胞凋亡特征,探讨滋养细胞凋亡现象与子痫前期病因及病理生理学之间的关系-方法:采用电镜扫描-原位细胞凋亡标记(TUNEL)及流式细胞仪技术,以正常妊娠妇女胎盘滋养细胞为对照,检测重度子痫前期患者胎盘组织中的滋养细胞凋亡-结果:①电镜下,重度子痫前期患者胎盘滋养细胞的超微结构均存在细胞核形态不规则-核膜固缩-异染色质异常分布-细胞质内线粒体基质变深-嵴模糊或排列紊乱-滋养细胞空泡化等凋亡形态学变化;②TUNEL法检测发现,重度子痫前期患者胎盘组织中存在较多凋亡滋养细胞,而正常胎盘组织中少见凋亡滋养细胞;③正常胎盘滋养细胞流式细胞检测图中凋亡细胞峰(Apo峰)不明显,重度子痫前期患者胎盘滋养细胞的流式细胞检测图中均存在较明显的亚二倍体DNA含量细胞峰(Apo峰)-结论:重度子痫前期患者胎盘存在滋养细胞凋亡特征,可能与子痫前期的发生发展密切相关-

    Abstract:

    Objective:To analysis the features of apoptosis in placental trophoblasts of patients with severe preeclampsia,and study the relationship between the trophoblastic apoptosis and the etiology and pathophysiology of preeclampsia. Methods:To obtain the evidences of apoptosis in the placental trophoblasts of patients with severe preeclampsia,the techniques of the electron microscope scanning,TUNEL and FCM were applied. Results:①In the ultrastructure of severe preeclamptic placental trophoblasts under the electron microscope scanning,there also were typic morphologic changes of apoptosis including anomalo-nucleus,pyknosis,abnormal distribution of heterochromatin,deeply-stained mitochondrial matrix in cytoplasm,clouding or disarrangement of mitochondrial crest,vacuoles in placental trophoblast cells,and so on. ②Under the detection of TUNEL technique,it was found that there were more numbers of apoptotic trophoblasts in the placenta of severe preeclampsia with fewer apoptotic cells appearing in the placenta of NC. ③In normal placental tissues,no markedly apoptotic peak(Apo peak) was found in the diagrams of FCM. However,there all were the markedly apoptotic peaks in the FCM diagrams of the severe preeclamptic placental tissues. Conclusion:Trophoblstic apoptosis in the placenta may contribute to the etiology or pathophysiology of preeclampsia.

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孙丽洲,杨娜娜.重度子痫前期患者胎盘滋养细胞凋亡现象的研究[J].南京医科大学学报(自然科学版),2007,(12):1436-1439

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  • 收稿日期:2007-01-28
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