芍药苷抑制NF-κB的活性促进人胃癌细胞凋亡
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江苏省医学重点人才135工程资助项目(522001)


Paeoniflorin induces the apoptosis of human gastric carcinoma cells by inhibiting activation of NF-κB
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    目的:探讨中药白芍的有效成分——芍药苷对人胃癌SGC-7901细胞株NF-κB的活性以及细胞凋亡的影响-方法:将芍药苷以不同浓度及不同时间作用于胃癌SGC-7901细胞-应用Western Blot方法检测各组胞核NF-κB表达情况,并应用胞核NF-κB活性检测(ELISA法)进一步确定胞核内NF-κB的表达情况,流式细胞仪检测联合应用芍药苷及5-氟尿嘧啶(5-FU)对胃癌细胞凋亡的影响-结果:芍药苷对胃癌SGC-7901细胞胞核NF-κB表达有明确的抑制作用,Western Blot以及胞核NF-κB活性检测结果均显示这种抑制存在着时间及剂量依赖现象,流式细胞术检测结果表明芍药苷对5-FU诱导的肿瘤细胞凋亡有明显的促进作用-结论:芍药苷抑制了细胞内NF-κB的表达,并借助这种作用增强5-FU诱导的细胞凋亡效应-

    Abstract:

    Objective:To investigate Paeoniflorin’s inhibition effect on NF-κB in human gastric carcinoma cells and its role in the induction of cell apoptosis. Methods:Paeoniflorin was diluted to different concentrations and treated on the gastric carcinoma cells at different time. Western Blot was used to test the expression of NF-κB in nuclear and cytoplasm. The intranuclear expression of NF-κB was confirmed by ELISA assay. The influence on cell apoptosis of gastric carcinoma cell by Paeoniflorin and 5-FU was estimated by flow cytometry. Results:Paeoniflorin dramaticly inhibitied NF-κB activity in nuclear and cytoplasm. The inhibition pattern of NF-κB exhibited in a time and dose dependence,which was confirmed by Western Blot and ELISA. Moreover,Paeoniflorin had a promotion on cell apoptosis induced by 5-FU. Conclusion:Paeoniflorin could inhibit NF-κB activity in nuclear and cytoplasm of SGC-7901 cells,and enhance cellular apoptosis of gastric carcinoma.

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吴 昊,李 薇,葛红梅,刘 平.芍药苷抑制NF-κB的活性促进人胃癌细胞凋亡[J].南京医科大学学报(自然科学版),2008,28(2):161-165

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  • 收稿日期:2007-09-12
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