Objective:To study the antitumor efficiency of Tetrandrine and Cisplatin on transplanted hepatocellular carcinoma(HCC) in mice. Methods:The model of transplanted HCC was established in mice. The tumor volume and weight changes of mice were observed. Results:Tetrandrine could inhibit the growth of the tumor. The inhibition rates were 40.1% and 47.2%, when concentration of Tetrandrine were 5 mg/kg and 10 mg/kg,respectively. The inhibition rates in the two groups were significantly different from that in the control group(P < 0.05). The inhibition rates were 55.8% and 59.9% when the concentration of Cisplatin were 2.5 mg/kg and 5 mg/kg,respectively;however,when combined with 5 mg/kg Tetradrine,the inhibition rates were 73.6%(P = 0.07) and 81.9%(P < 0.05), respectively;when combined with 10 mg/kg Tetrandrine,the inhibition rates were 82.3%(P = 0.06) and 91.4%(P < 0.01). The enhanced anti-cancer efficacy of Cisplatin by Tetradrine was observed in tumor-bearing mice,and had no obvious toxicity reaction. Conclusion:Both Tetrandrine and Cisplatin could inhibit the growth of the transplanted HCC in mice. Tetrandrine could enhance the antitumor effects of Cisplatin. Tetrandrine has synergistic effect with Cisplatin in the treatment of cancer.