Objevtive:To explore the gene expressive changes and related genes associated with colorectal cancer and hepatic metastases with gene microarray. Methods:The fresh specimens of primary cancer lesion and hepatic metastasis were collected in 5 patients suffered from colorectal cancer with liver metastasis during operation. After the total RNA extraction,the synthesized biotinylated cRNAs were hybridized to Agilent human 1A oligo microarray. After the design and synthesis of the primers,10 of the differentially expressed genes were then chosen at random to confirm the array results using the SYBR Green Real-time RT-PCR method in 21 fresh specimens collected from primary cancer lesion and hepatic metastasis.The data analysis depended on the ABI Prism 7500 system. Results:Using an extrance limit of ≥2.0 or ≤0.5,there were 110 up-regulated and 96 down-regulated genes identified by microarray which were commonly differentially expressed among the 5 cases. The array results were further confirmed by the real-time RT-PCR. Conclusion:Many genes were involved in the colorectal cancer with liver metastases. It could be helpful to find the regularity of the gene expression by gene microarray and to study the molecular mechanism in the colorectal cancer with liver metastases.