Objective:To explore the abundance of recombinant proteins expressed by the pET/E.coli system from different HCV gene regions. Methods:The four recombinant plasmids(pET-C\pET-NS5\pET-NS3-1\pET-NS3-6) were all transformed into the E.coli BL21(DE3) and screened by Kanamycin(50 -滋g/ml). After induced by isopropyl-β-D-thiogalactopyranoside(IPTG),the four expression products of fusion proteins were detected by 15% sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis(PAGE),and were purified by Ni-NTA Resin chromalography. The absorbances of the four recombinant proteins were measured by spectro photometer,and then the every protein concentration was calculated,respectively. Results:The four recombinant protein concentrations were 3.557(NS3 type 1),4.238(NS3 type 6),2.087(NS5) and 1.490 g/L(C),respectively. Conclusion:The result indicated that the abundance of four recombinant proteins was apparently different,derived by pET/E.coli system with different HCV gene fragments in the same condition.