吡格列酮对肥胖小鼠脂肪细胞因子脂联素表达的影响
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国家自然科学基金资助项目(NO30570878)


Effects of Pioglitazone on the adipocytokines in mice with diet-induced obesity
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    目的:通过构建饮食诱导肥胖小鼠模型,观察吡格列酮对脂肪细胞因子脂联素表达的影响,探讨过氧化物酶体增殖物激活受体(PPAR)-γ激动剂在胰岛素抵抗及2型糖尿病中的作用机制-方法:对肥胖组小鼠予吡格列酮(每日10 mg/kg)灌胃14天后处死,放免法测小鼠空腹胰岛素水平,生化法检测血糖,ELISA法检测血清脂联素含量,实时定量PCR检测脂肪组织脂联素的mRNA表达-结果:肥胖组小鼠体重,空腹胰岛素及血糖水平明显升高(P < 0.05)-给药后,肥胖干预组小鼠空腹胰岛素降低(P < 0.05),血糖降低(P < 0.01),血清脂联素含量升高(P < 0.01),HE染色显示脂肪细胞体积明显缩小,脂肪组织脂联素mRNA表达升高(P < 0.05)-结论:PPAR-γ激动剂通过影响脂肪细胞因子的表达,从而改善胰岛素抵抗-

    Abstract:

    Objective:To investigate the mechanism of Pioglitazone(PGZ) on insulin resistance and type 2 diabetes mellitus by using the DIO(diet-induced obese) mice model. Methods:The C57BL/6J mice were randomly divided into the normal diet group and high-fat diet(HFD) group. After 20 weeks,the obese mice were randomly divided into obese control group,PGZ group. They were orally administered placebo and PGZ[10 mg/(kg·d)] respectively for two weeks. The adiponectin mRNA expression levels from adipose tissue were analyzed by real-time quantitative PCR. The levels of plasma glucose,serum insulin and adiponectin were measured by Biochemistry technology,Radioimmunity and ELISA. The adipocyte sizes were also observed by Immunohistochemistry. Results:The weight,plasma glucose and serum insulin levels increased(P < 0.05) in HFD group,and the adipocyte sizes were bigger. Comparing to obese controls,serum insulin and glucose levels significantly decreased(P < 0.05) and the adipocyte sizes reduced in PGZ treatment group, while plasma adiponectin level raised(P < 0.01). Moreover,the mRNA expressions of adiponectin upregulated(P < 0.05). Conclusion:The effects of PPAR-γ agonist on insulin resistance can be carried out through regulating the expression of adipocytokines.

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谢 宇,朱 亭,刘 娟,钟 毅,丁国宪.吡格列酮对肥胖小鼠脂肪细胞因子脂联素表达的影响[J].南京医科大学学报(自然科学版),2008,28(7):872-875

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  • 收稿日期:2008-01-16
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