阿司匹林改善2型糖尿病患者血小板上内皮型一氧化氮合酶的功能
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江苏省自然科学基金(BK2005145),江苏省国际合作基金(BZ2006057),江苏省教育厅自然科学基金(06KJD310127),南京医科大学科技发展重点项目(06NMUZ006)


Aspirin improves the function of endothelium-type nitric oxide synthase in platelets of Type 2 diabetic patients
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    目的:探讨阿司匹林对2型糖尿病患者血小板上内皮型一氧化氮合酶(endothelium-type nitric oxide synthase,eNOS)功能的影响-方法:分别用浓度为25-50-100 μmol/L阿司匹林处理正常人和2型糖尿病患者的血小板,用比浊法测定血小板聚集率;根据L-[3H]-精氨酸在eNOS的作用下转化为L-[3H]-瓜氨酸的原理检测eNOS活性;用试剂盒检测cGMP的含量-结果:与对照组相比,阿司匹林可明显降低正常人和2型糖尿病患者的血小板聚集率,增强eNOS的活性,增加cGMP的含量,并且随着阿司匹林浓度的增加,其作用更明显(差异有显著性)-而同一浓度的阿司匹林对正常人血小板的作用比对2型糖尿病患者的作用更加明显-结论:阿司匹林可以浓度依赖性的改善2型糖尿病患者血小板上eNOS功能-

    Abstract:

    Objective:To investigate the effect of aspirin on the function of endothelium-type nitric oxide synthase(eNOS) in platelets of type 2 diabetic patients. Methods:Platelets,isolated from blood of healthy human subjects and type 2 diabetic patients,were exposed in vitro to aspirin at three different concentrations(25,50,100 μmol/L) and vehicle respectively. Platelets aggregation was measured turbidimetrically with a NBC Hema Tracer 601 aggregometer. In platelets,eNOS activity was determined by the ratio of the conversion of L-[3H]-arginine to L-[3H]-citrulline. Intraplatelet cGMP,an index of bioactive NO,was measured by radioimmunoassay. Results:Compared with the vehicle,aspirin concentration-dependently decreased the platelets aggregation,increased the activity of eNOS in platelets and intraplatelet cGMP level significantly,both in healthy human control and type 2 diabetic patients. And the effect was greater in healthy human subjects than that in type 2 diabetic patients at the same concentration of aspirin. Conclusion:Aspirin can improve the function of the platelet eNOS in type 2 diabetic patients in a concentration-dependent manner.

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崔伯塔,米琼宇,谢利平,杨 帆,黄 艳,季 勇.阿司匹林改善2型糖尿病患者血小板上内皮型一氧化氮合酶的功能[J].南京医科大学学报(自然科学版),2008,28(7):914-917

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  • 收稿日期:2008-04-09
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