白藜三醇对培养豚鼠心室肌细胞L-型钙电流密度的影响
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江苏省高校自然科学研究项目(05KJB320085)


Effects of resveratrol on the density of L-type calcium current in cultured ventricular myocytes of guinea pig
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    摘要:

    目的:观察白藜三醇(RES)对培养的豚鼠心室肌细胞L-型钙电流(ICa-L)密度的影响-方法:分离成年豚鼠心室肌细胞,采用全细胞膜片钳技术,记录不同浓度RES孵育心室肌细胞不同时间的ICa-L密度-结果:①培养72 h时,RES 30 μmol/L和100 μmol/L使ICa-L密度分别下降13%和27%,呈现浓度依赖性抑制趋势,且RES 100 μmol/L组与对照组比较有统计学差异(P < 0.05);②RES 10 μmol/L,30 μmol/L和100 μmol/L对 ICa-L密度呈现时间依赖性抑制趋势,且RES 100 μmol/L在24 h和72 h之间有统计学差异(P < 0.05);结论:白藜三醇对培养豚鼠心室肌细胞L-型钙电流呈现浓度和时间依赖性抑制趋势-

    Abstract:

    Objective:To investigate the effect of resveratrol on the density of L-type calcium current(ICa-L) in cultured guinea pig ventricular myocytes. Methods:Single cultured ventricular cells were obtained from healthy adult male guinea pig heart using enzymatic dissociation methods. The whole-cell patch clamp was used to record the ICa-L after administration of resveratrol at 24 h and 72 h with different final concentration of 0,10,30,100 μmol/L. Results:①30 μmol/L and 100 μmol/L RES decreased the density of ICa-L by 13% and 27%,respectively,at 72 h. There is a significant difference between control group and RES 100 μmol/L group(P < 0.05);②RES 10 μmol/L,30 μmol/L and 100 μmol/L have a tendency of reducing the density of ICa-L in a time-dependent manner. 100 μmol/L RES reduced ICa-L more significantly at 72 h compared with that did at 24 h(P < 0.05). Conclusion:Resveratrol have a tendency of inhibiting the density of L-type calcium current in cultured guinea pig ventricular myocytes at a concentration-and time-dependent manner.

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张志华,徐东杰,王 森,季建国,邹建刚,曹克将.白藜三醇对培养豚鼠心室肌细胞L-型钙电流密度的影响[J].南京医科大学学报(自然科学版),2008,28(8):964-967

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  • 收稿日期:2008-03-27
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