Objective:The casting sample of rat liver was fabricated, and the data of every section was harvested. The 3D-reconstruction of digital model of portal veins was reconstructed with computer and software. Methods:The propylene pigment preparation was injected into the rat portal vein(20 cases). The sample was embedded and cut into frozen sections. The data of every section of rat liver was collected by digital camera. Image segmentation and 3D-reconstruction were applied in photos. Results:Eighteen cases of the casting sample of rat liver were made successfully by this method. The propylene pigment preparation can enter small blood vessel whose diameter was 50~100 -滋m. Every case had about 200~300 photographs of the sections by an interval of 50 -滋m. They can be showed by computer vision based three-dimensional reconstruction. Conclusion:The method described above is feasible to get experimental data for 3D-reconstruction of digital model of rat liver and portal veins.