肾透明细胞癌中转化生长因子-β1?基质金属蛋白酶 2表达及其临床意义的相关性研究
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南京医科大学科技发展基金资助项目(07NMUM094)


The relative study of the expression of TGFβ-1 and MMP2 in renal clear cell carcinoma and their clinical significance
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    摘要:

    目的:研究转化生长因子-β1(TGF-β1)-基质金属蛋白酶 2(matrix metalloproteinases 2,MMP2)在肾透明细胞癌(renal clear cell carcinoma,RCCC)中的表达及临床意义-方法:采用免疫组织化学SP法测定80例RCCC及30例癌周组织中TGF-β1与MMP2的表达,同时采用逆转录-多聚酶链反应(RT-PCR)测定43例RCCC及43例癌周组织中TGF-β1与MMP2基因的表达-结果:RCCC中TGF-β1-MMP2表达强度均明显高于癌周组织,TGF-β1-MMP2表达与RCCC病理分级-临床分期相关;RCCC中TGF-β1与MMP2表达强度具有相关性;单因素生存分析显示TGF-β1-MMP2高表达与患者预后不良有关(P均 < 0.05)-结论:TGF-β1-MMP2基因的表达参与了RCCC的发展,并且二者之间具有一定的相关性,提示可以作为判断RCCC预后的二个有效的联合分子指标-

    Abstract:

    Objective:To investigate the expression of transforming growth factor beta 1(TGF-β1) and matrix metalloproteinases 2(MMP2) in human renal clear cell carcinoma(RCCC) and their clinical significance. Methods:The protein expression of TGF-β1 and MMP2 in 80 cases of RCCC and 30 cases of tissues adjacent to cancer were determined with immunohistochemical SP method. The mRNA expression of TGF-β1 and MMP2 gene in 43 cases of RCCC and 43 cases of tissues adjacent to cancer were determined with reverse transcription-polymerase chain reaction(RT-PCR). Results:In RCCC group TGF-β1 and MMP2 expression intensity was obviously higher than that in tissues adjacent to cancer. The expression of TGF-β1 and MMP2 were correlated with the pathologic grade and clinical stage of RCCC. There was a statistically significant correlation between expressions of TGF-β1 and MMP2. The high expressions of TGF-β1 and MMP2 significantly correlated with the poor prognosis of patients with RCCC by univariate survival analysis(P < 0.05,respectively). Conclusion:Up-regulation of TGF-β1 and MMP2 expression may be involved in the progression of RCCC. The correlation was found between TGF-β1 and MMP2. This indicates that TGF-β1 and MMP2 can be used as a useful associated-molecular marker for prognosis of RCCC.

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杨树东,孙荣超,穆会君,徐卓群,范钦和,张丽华,吴光斌,梁加贝,陈 瑛,芮 俊.肾透明细胞癌中转化生长因子-β1?基质金属蛋白酶 2表达及其临床意义的相关性研究[J].南京医科大学学报(自然科学版),2008,28(8):1037-

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  • 收稿日期:2007-12-29
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