Objective:To investgate the inhibiting effect of Weining granule on the growth and metastasis of orthotopic human gastric carcinoma. Methods:To establish the model of orthotopic human gastric carcinoma,60 rats with orthotopic human gastric carcinoma were randomly divided into the control group,CTX group,Weining granule group,CTX with Weining granule group,12 in each group. The inhibiting rate of carcinoma’s volume and weight,the change of shape on carcinoma cells,the expression of apoptotic index(AI),p53,Bcl-2,bax,CD44v6 and PCNA were all measured. Results:The inhibiting rate of carcinoma’s volume and weight in all CTX group,Weining granule group,CTX with Weining granule group were different from that of the control group(P < 0.05); the inhibiting rate of carcinoma’s volume and weight in CTX with Weining granule group were different from that of the CTX group(P < 0.05); the rate of hepatic metastasis in CTX group and Weining granule group were less than that of the control group(P < 0.05,P < 0.01);AI,p53,bcl-2,bax,CD44v6 and PCNA expression in Weining granule group were significantly different from that in the control group(P < 0.05);p53,CD44v6 and PCNA expression in CTX with Weining granule group were less than that in the CTX group(P < 0.05). Conclusion:Weining granule can inhibit the growth and metastasis of orthotopic human gastric carcinoma.