Objective:To explore the clinical Value of different-time 99Tcm-MIBI SPECT/CT fusion imaging for the diagnosis of the lung cancer. Methods:Sixty five patients with pulmonary abnormal lesions were chosen for performing different-time 99Tcm-MIBI SPECT/CT fusion imaging. They were divided into lung cancer and benign groups according to the pathologic results. Then the results were analyzed. The uptake ratio(UR) was calculated. Finally the diagnostic efficacy of different-time 99Tcm-MIBI SPECT/CT fusion imaging for the lung cancer was evaluated by the threshold of x+1s of the lung benign group. Results:The UR value of 45 cases in lung cancer group was 2.56 ± 0.53 at the time of 60 min SPECT/CT fusion imaging,while 20 cases of benign group was 1.47 ± 0.42. There was a significant UR difference in two groups(P < 0.05). Using the x + 1s of benign group UR value as a threshold,the diagnostic sensitivity,specificity and accuracy of different-time 99Tcm-MIBI SPECT/CT fusion imaging for the lung cancer was 86.67%,85.00% and 86.15% respectively. The false positive and false negative rate were 15.00% and 13.33%. The positive and negative predictive value were 92.86% and 73.91%. Conclusion:Different-time 99Tcm-MIBI SPECT/CT fusion imaging may be used in the diagnosis of lung cancer with great value and it could provide the important clinical information for clinical treatment planning.