观察氟伐他汀抑制血管紧张素Ⅱ诱导的肾小管上皮细胞增殖的影响
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江苏省卫生厅科技发展基金(H200317)


Effects of fluvastatin on proliferation of human renal tubular epithelial cell induced by Angiotensin Ⅱ
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    摘要:

    目的:观察氟伐他汀对血管紧张素Ⅱ(AngⅡ)诱导的肾小管上皮细胞增殖的影响. 方法:①不同浓度(10-9-10-8-10-7 -10-6-10-5 mol/L)AngⅡ刺激体外培养的肾小管上皮细胞;②固定浓度AngⅡ(10-5 mol/L)刺激,不同浓度(10-9-10-8-10-7-10-6-10-5 mol/L) 氟伐他汀干预;③在不同的时间点(24-48-72 h)用苯唑氮蓝法(MTT)检测细胞增殖-结果:①AngⅡ促进肾小管上皮细胞增殖,作用48 h增殖最明显,随着浓度增加,增殖作用增加,以10-5mol/L效果显著;②氟伐他汀能够显著抑制AngⅡ的促增殖作用,同样作用48 h抑制作用最明显,随着浓度增加,抑制作用增加,10-5 mol/L效果显著-结论:氟伐他汀能抑制AngⅡ诱导的肾小管上皮细胞增殖作用,48 h作用最强,且具有一定的浓度依赖性-

    Abstract:

    Objective:To investigate the effects of fluvastatin on proliferation of human renal tubular epithelial cell induced by AngiotensinⅡ. Methods:Cultured human renal tubular epithelial cells were treated with AngⅡ at different concentrations(10-9,10-8, 10-7,10-6 and 10-5 mol/L respectively) for different time(12 h,24 h,48 h). Then the cells were treated with AngⅡ(10-5 mol/L) and fluvastatin in different concentrations(10-9,10-8,10-7,10-6 and 10-5 mol/L respectively) for different time(12 h,24 h,48 h). The proliferation of renal tubular epithelial cells were determined by MTT colorimetry. Results:After treated with AngⅡ for 48 h,the proliferation of renal tubular epithelial cells was increased significantly, especially in 10-5 mol/L of AngⅡ, and then decreased significantly after treated with Fluvastatin,especially at the concentration of 10-5 mol/L for 48 h. Conclusion:Fluvastatin can inhibit the proliferation of human renal tubular epithelial cell induced by AngⅡ in a dose-dependent manner.

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储腊萍,刘 佳,王宁宁,王笑云.观察氟伐他汀抑制血管紧张素Ⅱ诱导的肾小管上皮细胞增殖的影响[J].南京医科大学学报(自然科学版),2008,28(11):1385-1388

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  • 收稿日期:2008-05-19
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