Objective:To evaluate the clinical appilication of contrast-enhanced ultrasound(CEUS) in the diagnosis of prostate cancer. Methods:Twenty-four prostate cancer patients confirmed by transrectal biospy with 32 nodules in the outer gland underwent CEUS with a bolus injection of SonoVue. The enhancement appearance of all the nodules were overviewed and the time-intensity curves(TIC) were drawn to record and analyze the parameters of contrast agent arrival time(AT),time to peak intensity(TTP) and peak intensity(PI). Results:Twenty-seven,2,2 and 1 of 32 nodules showed increased,equal,decreased and no enhancement,respectivery, compared with surrounding outer gland tissue. The AT and TTP of 31 enhancement nodules(16.9 ± 6.1)s and(29.1 ± 9.7)s,respectively were lower than that of the normal outer gland tissues(20.2 ± 6.6)s and(35.0 ± 7.9)s, respectively (P < 0.05),and the PI(9.6 ± 4.0)dB higher than that of the normal(7.5 ± 3.8)dB (P < 0.05). Conclusion:CEUS could reveal the presence of vasculature within the lesions of prostate cancer objectively and guide the prostate biopsy and TIC could be used in the quantity analysis of the lesions. CEUS could be helpful in the early diagnosis of the prostate cancer.