Objective:To investigate the inhibitory effects of As2O3 and Aspirin on cell proliferation in human gastric carcinoma cells(SGC-7901 cell lines). Methods:The inhibition of proliferation,apoptosis and cell cycle of gastric cancer cells SGC-7901 were detected by MTT assay and the flow cytometry and light microscope. Results:①After 72 hours,MTT assay showed the growth of the SGC-7901 cells was inhibited by As2O3(4 μmol/L), Aspirin(2 mmol/L),and As2O3+Aspirin(2 μmol/L + 1 mmol/L). And the inhibitory rates were 31.04%,16.11% and 24.19% respectively. It also showed the inhibitory effects among negative control group,two single drug groups(As2O3 group,Aspirin group) and drug combination group(P < 0.05). ②Flow cytometry showed the apoptosis rates were 1.55% in negative group,1.81% in As2O3 group,1.64% in Aspirin group and 19.01% in As2O3+Aspirin group,which showed statistical significance(P < 0.05). ③The proportion of cells at G0/G1 phase was significantly decreased while the proportion of cells at G2/M phase was increased in As2O3 group. The proportion of cells at G0/G1 phase was significantly increased while the proportion of cells at S phase and G2/M phase were decreased in Aspirin group. And the increasing of proportion of cells at G0/G1 phase and G2/M phase were found in drug combination group,while proportion of cells at S phase decreased. Conclusion:As2O3 and Aspirin could inhibit the growth of gastric cancer cell SGC-7901 by inducing apoptosis and cell cycle arrest. It showed that inhibitory effect on proliferation of SGC-7901 cell line in drug combination group(As2O3+Aspirin) were markedly enhanced and their dose was decreased.