ZnO纳米对雌性ICR小鼠急性毒性作用的研究
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973子课题(2001CCA04900),江苏省"科教兴卫工程"基金资助项目(LJ200606)


Effects of Zinc Oxide nanoparticles on the main organs in female mice:study in vivo
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    摘要:

    目的:研究氧化锌(ZnO)纳米颗粒对雌性小鼠的在体毒性作用,着重观察其对雌性小鼠生殖系统的影响-方法:成年雌性小鼠隔日腹腔注射ZnO纳米(200-500 mg/kg),对照组注射等体积生理盐水,共给药5次-停药1周后,测量各重要脏器的脏器系数,血清生化指标-雌二醇和孕激素的水平,主要脏器作病理切片和HE染色-结果:与对照组相比,给药ZnO纳米(200 mg/kg-500 mg/kg)的小鼠,肝脏-肾脏和卵巢的脏器系数均显著增加,200 mg/kg组的心脏脏器系数显著增加(P均 < 0.05);给药200 mg/kg和500 mg/kg组小鼠血生化指标中,尿酸(UA)显示增高,且有统计学意义(P < 0.05),提示在200~500 mg/kg ZnO纳米作用下肾功能有异常;此外,给药200 mg/kg和500 mg/kg组小鼠的乳酸脱氢酶(LDH)增高明显,提示心肌也有轻度的损伤-给药200 mg小鼠的雌二醇-孕酮水平没有统计学差异(P > 0.05),生殖系统脏器组织病理也未发现明显异常-结论:200~500 mg/kg ZnO纳米对雌性小鼠肝-肾和心功能有轻度影响,对小鼠的雌孕激素的分泌及卵巢组织无明显影响-因此,200~500 mg/kg ZnO纳米对小剂量ZnO纳米有一定的毒性,而对雌性小鼠生殖系统功能是安全的-

    Abstract:

    Objectives:To investigate in vivo the effects of zinc oxide(ZnO) nanoparticles on the main organs of female mice, especially on female reproductive system. Methods:Female mice, aged 5 weeks, were intraperitoneally injected with 200 and 500 mg/kg of zinc oxide(ZnO) nanoparticles, respectively, every other day for 5 times. Control group was injected with equal volume of normal saline. After one week of treatment, the coefficients of organs serum biochemical parameters and gonadal hormone(E2 and P) in female mice intraperitoneally treated with ZnO nanoparticles were measured. Main organs were observed in pathology. Results:Compared with control group, the coefficients of kidneys and ovaries were significantly changed in both 200 mg/kg group and 500 mg/kg group, and the coefficients of heart was significantly changed in 200 mg/kg group. The changes of coefficients of liver(P < 0.05) suggested the small hepatic injury after treatment with ZnO nanoparticles. UA in both groups raised significantly(P < 0.05), showing that ZnO nanoparticles had small nephrotoxicity after treatment with ZnO nanoparticles. The significantly change of serum LDH in both groups(P < 0.05) suggested the limited myocardial damage compared with the control group. However, there were no significant changes in pathological observation of main organs, including ovaries and uterus. The level of E2 and P were not significantly changed by the treatment with two doses of ZnO nanoparticles. Conclusions:There were slight damage in the function of liver, kidneys and heart with large dose of ZnO nanoparticles. However, there were no significant effect on the levels of E2 and P and pathology of ovaries. In conclusion, ZnO nanoparticles under 500 mg/kg dose was safe for reproductive system.

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刘晓慧,郭利利,秦定霞,高 莉,杨明德,张红梅,崔毓桂,刘嘉茵. ZnO纳米对雌性ICR小鼠急性毒性作用的研究[J].南京医科大学学报(自然科学版),2009,29(2):141-146

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  • 收稿日期:2008-10-10
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