Objective:To analyze the pathogen distribution and resistance in ICU of our hospital so as to provide the evidence for treatment. Methods:The bacteria and fungi were identified by API system; the sensitivity of antimicrobial agents and antifungal agents was tested by K-B and Rosco disk diffusion method; the data was analyzed by WHONET 5.4. Results:In 2007,there were 3341 specimens in our hospital including 343 specimens in ICU, and the positive rate of specimens was 7.7%. Gram-negative bacteria(70.7%) was the mains in ICU. Among the total, the rate of Nonfermenters was 46.9% and Enterobacteriaceae was 23.8%. Most of Nonfermenters were P. aeruginosa(37.3%) and Acinetobacter(7.6%). The majority of Gram-positive bacteria were Staphylococcus aureus. In view of the resistance, Staphylococcus species resistant to Vancomycin, while they were resistant to many other antimicrobial agents. Gram-negative Enterobacteriaceae were fairly sensitive to carbapenems, cefoperazone/sulbactam and piperacillin/tazobactam. Among nonfermenters, P. aeruginosa were resistant to the most antimicrobial agents while Acinetobacter were comparatively sensitive to carbapenems and cefoperazone/sulbactam. Conclusion:The pathogens in ICU have a high resistant rate to many antimicrobial agents. In view of the seriousness of this problem, we must lay enough emphasis on it and select antimicrobial agents rational.